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DNA Engineered Noble Metal Nanoparticles: Fundamentals and State-of-the-Art of Nanobiotechnology

DNA Engineered Noble Metal Nanoparticles: Fundamentals and State-of-the-Art of Nanobiotechnology

Authors
Publisher John Wiley & Sons Inc
Year 2015
Pages 672
Version hardback
Readership level Professional and scholarly
Language English
ISBN 9781118072141
Categories Nanotechnology
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Book description

There is a growing interest in the use of nanoparticles modified
with DNAs, viruses, peptides and proteins for the rational design
of nanostructured functional materials and their use in biosensor
applications. The challenge is to control the organization of
biomolecules on nanoparticles while retaining their biological
activity as potential chemical and gene therapeutics. These
noble metal nanoparticles/biomolecules conjugates have specific
properties and therefore they are attractive materials for
nanotechnology in biochemistry and medicine.





In this book, the author review work performed dealing with the
DNA structure and functionalities, interactions between DNA, noble
metal nanoparticles, surface active agents, solvents and other
additives. Particular attention is given to how the DNA?s
chain length and the DNA conformation affect the interaction and
structure of the nanoconjugates and nanostructures that are formed.
Also discussed are the recent advances in the preparation,
characterization, and applications of noble metal nanoparticles
that are conjugated with DNA aptamers and oligomers. The advantages
and disadvantages of functionalized nanoparticles through various
detection modes are highlighted, including colorimetry,
fluorescence, electrochemistry, SPR, and, mass spectrometry for the
detection of small molecules and biomolecules. The functionalized
noble metal nanoparticles are selective and sensitive for the
analytes, showing their great potential in biosensing. Furthermore,
this book reviews recent progress in the area of DNA-noble metal
nanoparticles based artificial nanostructures, that is, the
preparation, collective properties, and applications of various
DNA-based nanostructures are also described.

DNA Engineered Noble Metal Nanoparticles: Fundamentals and State-of-the-Art of Nanobiotechnology

Table of contents

Acknowledgement ix Preface xi 1 Introduction 1 2 Nucleic Acids 29 2.1 DNA/RNA Basics 29 2.2 Aptamers, Telomers and Oligonucleotides 50 2.3 Techniques and Approaches 63 2.4 DNA-Based Molecular Nanomachines 90 2.5 Peptide Nucleic Acid 104 2.6 Nanobiotechnology 112 3 Noble Metal Nanoparticles 121 3.1 Preparation and Modification 121 3.2 Optical and Physical Properties 137 3.3 Conjugates 144 4 DNA-Based Conjugates 149 4.1 General 149 4.2 Condensation 152 4.3 Conjugates 170 5 DNA-Noble Metal Nanoparticle Conjugates 173 5.1 General Approaches 173 5.2 DNA Monomers and Oligomers 178 5.2.1 Gold Nanoparticles 178 5.2.2 Silver Nanoparticles 207 5.3 Hybridization and Denaturation 224 5.4 DNA Biotemplates 273 6 DNA-Gold Nanoparticle Conjugates 281 6.1 DNA-Gold Zero-Dimensional Nanoparticle Conjugates 281 6.2 DNA One-Dimensional Gold Nanoparticle Conjugates 295 7 PNA-Noble Metal Nanoparticles Conjugates 315 7.1 PNA-Gold Nanoparticle Conjugates 315 7.2 PNA-Silver Nanoparticle Conjugates 328 8 DNA-Silver Nanoparticles Conjugates 335 9 The Structure of DNA-Noble Metal Nanoparticles Conjugates 345 9.1 Configuration of DNA-Noble Metal Nanoparticles Conjugates 345 9.2 Stabilization of DNA Conjugates 358 9.3 Nanostructures and Nanoconstructs 369 9.4 Colorimetric and Sensing Assays 385 10 Photochemical and Photophysical Events 403 10.1 Noble Metal Nanoparticles 403 10.2 DNA Nucleobases 406 10.3 DNA/PNA 417 10.4 DNA-Dyes Conjugates 429 10.5 DNA-AuNP-Dye Conjugates 443 10.6 DNA-Gold Nanoparticle Conjugates 455 10.7 DNA-AgNPs 465 10.8 Hot Gold Nanoparticles 475 11 Nanoparticle Therapeutics 481 11.1 Biodecorated Nanoparticle-Based Therapies 481 11.2 Photothermal Therapy 489 11.3 Cells 495 11.4 Gene Therapy 499 11.5 Blood Fluid Effect 505 11.6 Other Application Approaches 507 12 Conclusion 515 Nomenclature 533 DNA Entities 545 Vocabulary and Definitions 549 References 564 Index

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