Bookstore

Inaddition to updating important core techniques this third edition presents newchapters on assessing leukocyte involvement in angiogenesis, lymphatic cell andpericyte isolation techniques, spheroid and arterial ring based in vitroassays, and on pericyte involvement in angiogenesis. Angiogenesis Protocols,Third Edition also guides readers through new protocols on chorioallantoicmembrane models, corneal pocket assays to assess angio- and lymphangiogenesis,models of muscle angiogenesis, and use of zebrafish embryos to study vascularangiogenesis and senescence. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.

Authoritativeand practical, Angiogenesis Protocols, Third Edition provides not only apractical handbook for key techniques, but also an informative and enjoyableread for all those interested, no matter how directly, in angiogenesis.

Angiogenesis Protocols

Part I: Microscopic Assessment

 

1.         Assessing TumorAngiogenesis in Histological Samples

Jia-Min Pang, Nicholas Jene, and Stephen B Fox

2.         Immunohistochemical Methodsfor Measuring Tissue Lymphangiogenesis

Daniel J. Royston, Steven Clasper, and David G. Jackson

3.         Immunohistochemical Assessmentof Leukocyte Involvement in Angiogenesis

Narmeen S. Ahmad, Stewart G. Martin, and Sarah J. Storr

 

Part II: Cell Isolation Techniques

 

4.         Isolation and Culture ofHuman Endothelial Cells from Micro- And Macro-Vessels

Peter W Hewett

5.         Isolation,Identification and Culture of Human Lymphatic Endothelial Cells

Zerina Lokmic

6.         Isolation, Culture andCharacterisation of Vascular Smooth Muscle Cells

Jessal J Patel and Richard CM Siow

7.         Isolation and Transfectionof Primary Culture Bovine Retinal Pericytes

Vincent A. Primo and Joseph F. Arboleda-Velasquez

 

Part III: In Vitro Techniques

 

8.         In Vitro Assays for Endothelial CellFunctions Required for Angiogenesis: Proliferation, Motility, TubularDifferentiation And Matrix Proteolysis

Suzanne A Eccles, William Court, and Lisa Patterson

9.         Tube Forming Assays

Ryan M. Brown, Christopher J. Meah, Victoria L. Heath, Iain B. Styles,and Roy Bicknell

10.       In Vitro Co-CultureAssays of Angiogenesis

Mark Richards and Harry Mellor

11.       Spheroid-Based In VitroAngiogenesis Model

Larissa Pfisterer and Thomas Korff

12.       Stem Cell Spheroid-Based Sprout Assay inThree-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study OfAngiogenesis

Fatemeh Sharifpanah and Heinrich Sauer

13.       Human Arterial RingAngiogenesis Assay

Giorgio Seano and Luca Primo

14.       A Modified Aortic RingAssay to Assess Angiogenic Potential In Vitro

Nina Zippel, Yindi Ding, and Ingrid Fleming

15.       Quantitative Imaging-Based Examination ofPericyte Control of Endothelial Growth Dynamics and Angiogenesis.

Anthony R. Sheets, Jennifer T. Durham, and Ira M. Herman

16.       Static and Dynamic Assaysof Cell Adhesion Relevant to the Vasculature

Lynn M. Butler, Helen M. Mcgettrick, and Gerard B. Nash

 

Part IV: In Vivo Techniques

 

17.       Dorsal Skinfold Chamber Preparation inMice: Studying Angiogenesis by Intravital Microscopy

Axel Sckell and Michael Leunig

18.       Quantitative Estimation ofTissue Blood Flow Rate

Gillian M Tozer, Vivien E Prise, and Vincent J Cunningham

19.       Chorioallantoic Membrane Microtumor Modelto Study the Mechanisms of Tumor Angiogenesis, Vascular Permeability, and TumorCell Intravasation

Elena I. Deryugina

20.       The Rabbit Corneal PocketAssay

Lucia Morbidelli and Marina Ziche

21.       The Corneal MicropocketAssay:  A Model of Angiogenesis andLymphangiogenesis

Shintaro Nakao and Ali Hafezi-Moghadam

22.       Models of Oxygen InducedRetinopathy in Rodents

Melissa V. Gammons and David O. Bates

23.       The Sponge Implant Model ofAngiogenesis

Silvia Passos Andrade and Mônica Alves Neves Diniz Ferreira

24.       Measurement of Angiogenesis,Arteriolargenesis and Lymphangiogenesis Phentotypes by Use of Two-DimensionalMesenteric Angiogenesis Assay

Andrew V. Benest and David O. Bates

25.       In Vivo Models of MuscleAngiogenesis

Stuart Egginton

26.       Use of the Hollow Fibre Assay to EvaluateAgents that Target the Tumor Neovasculature

Patricia A. Cooperand Steven D. Shnyder

27.       Studying Vascular Angiogenesis andSenescence in Zebrafish Embryos

Emma Ristori, Sandra Donnini, and Marina Ziche