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This book details sorghum breeding technologies, grain compounds, nutrition and digestibility, biotechnology methods, broad renewable applications and an economic study.  Chapters are divided into five review chapters, five case study chapters, and nine protocol chapters providing comprehensive reviews, new study results or state-of-the-art protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Sorghum: Methods and Protocols aims to provide useful information and tools to an array of readers looking to research and utilize sorghum.  

Sorghum: Methods and Protocols

Preface...Table of Contents...Contributing Authors...

Part I Breeding Technologies
1. Hybrid Sorghum Product Development and ProductionLarry Lambright
2. Mapping QTLs and Identification of Genes Associated with Drought Resistance in SorghumKaren R. Harris-Shultz, Chad M. Hayes, and Joseph E. Knoll
3. Field Studies to Develop Weed Management Programs for Grain SorghumJ. Anita Dille
4. Discovery of Sorghum Haploid Induction SystemTanveer Hussain and Cleve Franks
5. Development of a Pedigreed Sorghum Mutant LibraryJunping Chen, Guihua Zou, and Zhanguo Xin
6. Marker Assisted Selection in Sorghum using KASP Assay for Detection of Single Nucleotide Polymorphism/Insertion DeletionGloria Burow, Ratan Chopra, Halee Hughes, Zhanguo Xin, and John Burke
Part II Grain Compounds, Nutrition, and Digestibility
7. Assaying Sorghum Nutritional QualityKwaku G. Duodu
8. Tannin Analysis in Sorghum GrainsLinda Dykes
9. Sorghum Phytochemicals and their Potential Impact on Human HealthLinda Dykes
10. Identification and Quantification of Carotenoids and Tocochromanols in Sorghum Grain by High Performance Liquid ChromatographyDarwin Ortiz and Mario G. Ferruzzi
Part III Biotechnologies
11. Gene Editing in Sorghum through AgrobacteriumJeffry D. Sander
12. Genome Editing by CRISPR/Cas9 in Sorghum through Biolistic BombardmentGuoquan Liu, Jieqing Li, and Ian D. Godwin
13. Novel Ternary Vectors for Efficient Sorghum TransformationAjith Anand, Ping Che, Emily Wu, and Todd J. Jones
14. Nutritionally Enhanced Sorghum for the Arid and Semi-Arid Tropical Areas of AfricaZuo-yu Zhao, Ping Che, Kimberly Glassman, and Marc Albertsen
15. Evaluation of Agronomic Performance of beta-Carotene Elevated Sorghum in Confined Field ConditionsPing Che, Zuo-Yu Zhao, Mark Hinds, Kristen Rinehart, Kimberly Glassman, and Marc Albertsen
Part IV Economics and Broad Application
16. Economics of Increasing Sorghum Productivity in Sub-Saharan Africa: The Mali CaseJohn H. Sanders, Botorou Ouendeba, Ababacar Ndoye, Niaba Témé, and Soungalo Traore
17. Background on Grain Sorghum UsageJohn Duff, Austin Vincent, Doug Bice, and Ian Hoeffner
18. Assaying Sorghum for Fuel ProductionCourtney Payne, Justin Sluiter, and Ed Wolfrum
19. The Role of Sorghum in Renewables and BiofuelsJeff Dahlberg