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The volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems. The chapters in this book cover topics such as Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial siderophores analysis by mass spectrometry; the metabolomic study of tissues in Parkinson's Disease; and NMR analysis in livestock metabolomics. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, tips on troubleshooting and avoiding pitfalls.

Cutting-edge and thorough, Metabolomics: Methods and Protocols is an essential resource for any researcher interested in this exciting and evolving field. 

Metabolomics: Methods and Protocols

Preface...Table of Contents...Contributing Authors...
1.Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass SpectrometerLukás Najdekr, Giovanny Rodriguez Blanco, and Warwick B. Dunn
2.Isotopic Ratio Outlier Analysis (IROA) for Quantitative AnalysisChris Beecher and Felice de Jong
3.Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H SignalsMukundan Ragavan and Matthew E. Merritt
4.Quantitative Metabolomics using Isotope Residue Outlier Analysis (IROA®) with Internal StandardsRoberto Mendez, Maria del Carmen Piqueras, Alexander Raskind, Felice de Jong, Chris Beecher, Sanjoy Bhattacharya, and Santanu Banerjee 
5.Analyses of Cholesterol Metabolites of Optic Nerve using GC-MS MethodsGurkaran S. Sarohia, Erik D. Garza, and Sanjoy K. Bhattacharya
6.Analyses of Cholesterol and Derivatives in Ocular Tissues using LC-MS/MS MethodsMaria Piqueras, Despoina Theotoka, Gurkaran Sarohia, and Sanjoy K. Bhattacharya
7.Measurement of Pyridine Nucleotides in Biological Samples using LC-MS/MSChris Petucci, Jeffrey A. Culver, Nidhi Kapoor, Hampton Sessions, Daniela Divlianska, and Stephen J. Gardell
8.Handling and Analysis of 5-Formyl-, 5,10-Methenyl-, 10-Formyl-, 5-Formimno-, and 5,10-MethylenetetrahydrofolatesAnita H. Lewin and Peter Silinski
9.Protein-Lipid Complex Separation Utilizing a Capillary Electrophoresis System Anddre Osmar Valdivia, Ciara Myer, Maria Fernanda Suarez, and Sanjoy K. Bhattacharya
10.Immature and Mature Collagen Crosslinks Quantification using High Performance Liquid Chromatography and High Resolution Mass Spectrometry in OrbitrapTMDiana R. Hernandez, Maria del Carmen Piqueras, Alejandro E. Macias, Laisel Martinez, Roberto Vazquez-Padron, and Sanjoy K. Bhattacharya
11.Relative Quantitation of Neurotoxic Metabolites of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS)Pallavi Lahiri, Deepika Dhaware, Ajeet Singh, Venkat Panchagnula, and Dipankar Ghosh
12.Analysis of Microbial Siderophores by Mass Spectrometry Tomas Pluhacek, Anton Skriba, Jiri Novak, Dominika Luptakova, and Vladimir Havlicek
13.HSD18B7 Enzyme Assay Technique using a Triple Quadrupole Mass SpectrometerCarina Wu, Nhuong-Sao Ton, Maria Fernanda Suarez, Maria Piqueras Soria, and Sanjoy K. Bhattacharya
14.Assays for Intracellular Cyclic Adenosine Monophosphate (cAMP) and Lysosomal AcidificationChiranjit Maity, Dipankar Ghosh, and Sonia Guha
15.Isotopic Ratio Outlier Analysis (IROA) of Aqueous Humor for MetabolitesMaria Piqueras, Ciara Myer, Anna Junk, and Sanjoy K. Bhattacharya
16.Assessment of Transport of Lipid Metabolites within Trabecular Meshwork CellsDaphne Toglia, Stephanie Dancausse, Karthik Kalahasty, Bryan Alfonso, and Sanjoy K. Bhattacharya
17.Detection and Quantification of Lipid Droplets in Differentiated Human PodocytesShamroop Kumar Mallela, Devang Maheshkumar Patel, Gloria Michelle Ducasa, Sandra Merscher, Alessia Fornoni, Hassan Al-Ali
18.Labeling Cancer Stem Cells with 13C6 Glucose and 13C5 Glutamine for Metabolic Flux AnalysisKousik Kesh and Sulagna Banerjee
19.Protocols for Metabolomic Analyses from Tissues in Parkinson's DiseaseJade Woods, Shulei Lei, Jordan Rose,  Darrell D. Marshall, Fatema Bhinderwala, Eli Riekeberg, Aline De Lima Leite, Martha Morton, Eric D. Dodds, Rodrigo Franco, and Robert Powers
20.Application of Stable Isotope Labels for Metabolomics in Studies in Fatty Liver DiseasePatrycja Puchalska and Peter A. Crawford
21.Proteometabolomics of Melphalan Resistance in Multiple MyelomaDavid C. Koomen, Joy D. Guingab-Cagmat, Paula S. Oliveira, Bin Fang, Min Liu, Eric A. Welsh, Mark B. Meads, Tuan Nguyen, Laurel Meke, Steven A. Eschrich, Kenneth H. Shain, Timothy J. Garrett, and John M. Koomen
22.Metabolomic Analyses of Muscle Atrophy Induced by Hindlimb UnloadingStephen J. Gardell, Xiaolei Zhang, Nidhi Kapoor, Christopher Petucci, and Paul M. Coen
23. Protocols for NMR Analysis in Livestock MetabolomicsAidin Foroutan, Seyed Ali Goldansaz, Matthias Lipfert, and David S. Wishart
24. Metabolomics Applicable to Retinal Vascular DiseasesSatish Patnaik, Subhadra Jalali, Manjunath B. Joshi, Kapaettu Satyamoorthy, Inderjeet Kaur