As experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly became a necessity. Angiogenesis Protocols, Second Edition remains true to its original vision of providing a single source for angiogenesis researchers, irrespective of levels of resource and expertise, by collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro. This information, however, is expanded to include chapters on circulating endothelial progenitor cells, angiogenic signalling pathways, imaging of angiogenesis, and measurement of tissue blood flow. Written in the Methods in Molecular Biology(TM) series format, the chapters provide step-by-step laboratory protocols, lists of necessary materials and reagents, and a Notes section, which details tips on troubleshooting and avoiding known pitfalls.
Extensive and cutting-edge, Angiogenesis Protocols, Second Edition is not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis.
Angiogenesis Protocols
Table of contents
Section I: Review Articles
Chapter 1. Angiogenic Signalling Pathways
Zubair Ahmed and Roy Bicknell
Chapter 2. Imaging Angiogenesis
Natalie Charnley, Stephanie Donaldson, and Pat Price
Section II: Microscopic Assessment
Chapter 3. Assessing Tumor Angiogenesis in Histological Samples
Stephen B Fox
Chapter 4. Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis
Steven Clasper and David G. Jackson
Section III: Cell Isolation Techniques
Chapter 5. Vascular Endothelial Cells from Human Micro- and Macro-vessels: Isolation, Characterisation and Culture
Peter W Hewett
Chapter 6. Lymphatic Endothelial Cells: Establishment of Primaries and Characterization of Established Lines
Riccardo E. Nisato and Michael S. Pepper
Chapter 7. Isolation, Culture and Characterisation of Vascular Smooth Muscle Cells
Adrian T Churchman and Richard CM Siow
Chapter 8. Circulating Endothelial Progenitor Cells (CEPC)
Michael C. Schmid and Judith A. Varner
Section IV: In vitro Techniques
Chapter 9. In vitro Assays for Endothelial Cell Functions Related to Angiogenesis: Proliferation, Motility, Tubular Differentiation and Proteolysis
Suzanne A Eccles, William Court, Lisa Patterson and Sharon Sanderson
Chapter 10. Tube Formation: an In Vitro Matrigel Angiogenesis Assay
M. Lourdes Ponce
Chapter 11. Three-dimensional In vitro Angiogenesis in the Rat Aortic Ring Model
David C. West and Mike F. Burbridge
Chapter 12. Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature
Lynn M. Butler, Helen M. McGettrick, and Gerard B. Nash
Chapter 13. Genetic Manipulation of Corneal Endothelial Cells: Transfection and Viral Transduction
Eckart Bertelmann
Chapter 14. Creation of Human Skin Equivalents for the In vitro Study of Angiogenesis in Wound Healing
Ira M. Herman and Alice Leung
Section V: In vivo Techniques
Chapter 15. Measurement of Angiogenic Phenotype by Use of a Two Dimensional Mesenteric Angiogenesis Assay
Andrew V Benest and David O Bates
Chapter 16. Quantitative Estimation of Tissue Blood Flow Rate
Gillian M Tozer, Vivien E Prise and Vincent J Cunningham
Chapter 17. In Vivo Matrigel Migration and Angiogenesis Assay
Katherine M. Malinda
Chapter 18. The Sponge Implant Model of Angiogenesis
Silvia Passos Andrade and Mônica Alves Neves Diniz Ferreira
Chapter 19. The Dorsal Skinfold Chamber: Studying Angiogenesis by Intravital Microscopy
Axel Sckell and Michael Leunig
Chapter 20. The Corneal Pocket Assay
Marina Ziche and Lucia Morbidelli
Chapter 21. Use of the Hollow Fibre Assay for Studies on Tumor Neovasculature
Steven D. Shnyder
Chapter 22. The Cranial Bone Window Model: Studying Angiogenesis of Primary and Secondary Bone Tumors by Intravital Microscopy
Axel Sckell and Frank M. Klenke
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