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This book highlights recent advances in and diverse techniques for exploring the plasma membrane's structure and function. It starts with two chapters reviewing the history of membrane research and listing recent advances regarding membrane structure, such as the semi-mosaic model for red blood cell membranes and the protein layer-lipid-protein island model for nucleated tissue cell membranes. It subsequently focuses on the localization and interactions of membrane components, dynamic processes of membrane transport and transmembrane signal transduction. Classic and cutting-edge techniques (e.g. high-resolution atomic force microscopy and super-resolution fluorescence microscopy) used in biophysics and chemistry are presented in a very comprehensive manner, making them useful and accessible to both researchers in the field and novices studying cell membranes. This book provides readers a deeper understanding of the plasma membrane's organization at the single molecule level and opens a new way to reveal the relationship between the membrane's structure and functions, making it essential reading for researchers in various fields.

Membrane Biophysics

Composition and Function of Cell Membranes.- History and Traditional Techniques of Studying the Structure of Cell Membranes.- Imaging membranes by High resolution Atomic Force Microscopy.- Detection of Membrane Mechanical Properties and Endocytosis by Single Molecule Force Spectroscopy.- Super-resolution Imaging of Membrane Heterogeneity.- Analysis and Applications of Single Molecule Fluorescence in Live Cell Membranes.- Lipid Cubic Phase for Membrane Protein X-ray Crystallography.- Electron Microscopic Analysis of the Plasma Membrane and Cell Surface Molecules.- Solid-state Nuclear Magnetic Resonance Spectroscopy of Membrane Proteins.- Mass Spectrometry of Membrane Proteins.- Infrared Spectroscopy for Studying Plasma Membranes.- Computer simulations to explore membrane organization and transport.- Other Modern Methods for Studying Biomembranes.