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Culture of Animal Cells 6e

Culture of Animal Cells 6e

Autorzy
Wydawnictwo John Wiley & Sons
Data wydania 01/10/2010
Wydanie 6
Liczba stron 768
Forma publikacji książka w twardej oprawie
Poziom zaawansowania Dla profesjonalistów, specjalistów i badaczy naukowych
Język angielski
ISBN 9780470528129
Kategorie Biologia komórkowa (cytologia), Biotechnologia
378.24 PLN (z VAT)
$98.31 / €89.08 / £75.08 /
Produkt na zamówienie
Dostawa 3-4 tygodnie
Ilość
Do schowka

Opis książki

This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs. PowerPoint slides of the figures as well as other supplementary materials are available at a companion website: www.wiley.com/go/freshney/cellculture "The book's sixth incarnation includes new sections on organotypic culture and different cell and stem cell types". (Veterinary Times, 11 April 2011)

Culture of Animal Cells 6e

Spis treści

List of Figures. List of Color Plates. List of Protocols. Preface and Acknowledgements. Abbreviations. 1 Introduction. 1.1 Historical Background. 1.2 Advantages of Tissue Culture. 1.3 Limitations. 1.4 Major Differences In vitro. 1.5 Types of Tissue Culture. 2 Biology of Cultured Cells. 2.1 The Culture Environment. 2.2 Cell Adhesion. 2.3 Cell Proliferation. 2.4 Differentiation. 2.5 Cell Signaling. 2.6 Energy Metabolism. 2.7 Origin of Cultured Cells. 3 Laboratory Design, Layout, and Equipment. 3.1 Layout, Furnishing, and Services. 3.2 Layout. 4 Equipment and Materials. 4.1 Requirements of a Tissue Culture Laboratory. 4.2 Aseptic Area. 4.3 Incubation and Culture. 4.4 Preparation and Sterilization. 4.5 Storage. 4.6 Supplementary Laboratory Equipment. 4.7 Specialized Equipment. 5 Aseptic Technique. 5.1 Objectives of Aseptic Technique. 5.2 Elements of Aseptic Environment. 5.3 Sterile Handling. 5.4 Standard Procedure. 5.5 Apparatus and Equipment. 6 Safety, Bioethics, and Validation. 6.1 Laboratory Safety. 6.2 Risk Assessment. 6.3 Standard Operating Procedures. 6.4 Safety Regulations. 6.5 General Safety. 6.6 Fire. 6.7 Ionizing Radiation. 6.8 Biohazards. 6.9 Bioethics. 6.10 Quality Assurance. 6.11 Validation. 7 Culture Vessels and Substrates. 7.1 The Substrate. 7.2 Treated Surfaces. 7.3 Choice of Culture Vessel. 7.4 Specialized Systems. 8 Defined Media and Supplements. 8.1 Development of Media. 8.2 Physicochemical Properties. 8.3 Balanced Salt Solutions. 8.4 Complete Media. 8.5 Serum. 8.6 Selection of Medium and Serum. 8.7 Other Supplements. 9 Serum-Free Media. 9.1 Disadvantages of Serum. 9.2 Advantages of Serum-Free Media. 9.3 Disadvantages of Serum-Free Media. 9.4 Replacement of Serum. 9.5 Selection of Serum-Free Medium. 9.6 Development of Serum-Free Medium. 9.7 Preparation of Serum-Free Medium. 9.8 Animal Protein-Free Media. 9.9 Conclusions. 10 Preparation and Sterilization. 10.1 Preparation of Reagents and Materials. 10.2 Sterilization of Apparatus and Liquids. 10.3 Apparatus. 10.4 Reagents and Media. 10.5 Sterilization of Media. 10.6 Control, Testing, and Storage of Media. 11 Primary Culture. 11.1 Initiation of a Primary Cell Culture. 11.2 Isolation of the Tissue. 11.3 Types of Primary Culture. 12

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