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Understanding Light Microscopy

Understanding Light Microscopy

Autorzy
Wydawnictwo Blackwell Science
Data wydania 01/05/2019
Wydanie Pierwsze
Liczba stron 848
Forma publikacji książka w twardej oprawie
Poziom zaawansowania Dla profesjonalistów, specjalistów i badaczy naukowych
Język angielski
ISBN 9780470973752
Kategorie Biologia, Inżynieria mechaniczna i materiałowa
795.00 PLN (z VAT)
$210.03 / €174.57 / £150.81 /
Produkt dostępny
Dostawa 2 dni
Ilość
Do schowka

Opis książki

This textbook introduces readers to the modern light microscope. It can either be used alone or in conjunction with a practical course. The book begins with an account of how the light microscope works, and how to set up the instrument for optimum performance. The nature of light, and its influence on the function of the microscope, as well as practical advice on selecting the most suitable components, is explained to the reader. An account of the different types of currently-available light microscopy modes follows, together with a guide, borne out by the authors practical experience, on how to choose between transmitted or reflected light microscopes; wide-field, confocal, deconvolution or multi-photon microscopes. A description of specimen preparation is given, as well as advice upon cameras, image acquisition and post-processing of data. * Gives a didactic introduction to the light microscope * Explanation complemented with simple experiments where appropriate * Simple explanation (from teaching the subject) enables a self-taught student to start from scratch to set up and acquire high quality images * Leads the student on, should they wish, to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility * Well-illustrated in colour, the illustrations are selected (from long teaching experience) to support text * Workable practical protocols * Includes a chapter summary to introduce learning objectives Part of the Royal Microscopical Society co-publication book program

Understanding Light Microscopy

Spis treści

About the Author ix


Acknowledgements xi


Look-Up Guide to Feature Boxes by Theme xii


Glossary and Definitions xiv


Notes xxiv


Introduction xxvii


1 Our Eyes and the Microscope 1


2 Light 29


3 Basic Microscope Optics 55


4 Microscope Anatomy and Design 75


5 Ergonomics 91


6 Optical Aberrations of the Microscope 101


7 The Microscope Objective 127


8 Condensers and Eyepieces 161


9 Illumination in the Microscope 177


10 Diffraction and Image Formation in Microscopy 211


11 Contrast Generation and Enhancement 243


12 Reflected-Light Microscopy 289


13 Polarised-Light Microscopy: Part 1 - Theory 317


14 Polarised-Light Microscopy: Part 2 - Applied 347


15 Fluorescence Microscopy 383


16 Fluorophores and Fluorescent Proteins 405


17 Optical Sectioning and Confocal Microscopy 425


18 Operating the Confocal Microscope 447


19 Light-Sheet Microscopy 483


20 Bleed-Through and Spectral Unmixing 507


21 Deconvolution 523


22 Multi-Photon Microscopy 543


23 Total Internal Reflection Fluorescence Microscopy 561


24 FRAP and FRET 569


25 Colocalisation 587


26 Super-Resolution Fluorescence Microscopy 613


27 Choosing a Microscope Platform and Core Imaging Facilities 637


28 Biological Specimen Preparation 663


29 Materials Specimen Preparation 687


30 Recording the Image: Part 1 - Theory 707


31 Recording the Image: Part 2 - Applied 733


Appendices


1 Buying, and Tendering for, a Light Microscope 769


2 Troubleshooting Poor Image Quality 773


3 The Michel-Levy Interference Colour Chart 775


4 Cleaning and Maintenance of the Light Microscope 779


5 Selected Suppliers 783


6 Historical Background 787


7 Timeline of Key Events 791


Index 799

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