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Understanding Light Microscopy

Understanding Light Microscopy

Wydawnictwo Blackwell Science
Data wydania 01/05/2019
Wydanie Pierwsze
Liczba stron 848
Forma publikacji książka w twardej oprawie
Poziom zaawansowania Dla profesjonalistów, specjalistów i badaczy naukowych
Język angielski
ISBN 9780470973752
Kategorie Biologia, Inżynieria mechaniczna i materiałowa
795.00 PLN (z VAT)
$210.03 / €174.57 / £150.81 /
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Dostawa 2 dni
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Opis książki

This textbook introduces readers to the modern light microscope. It can either be used alone or in conjunction with a practical course. The book begins with an account of how the light microscope works, and how to set up the instrument for optimum performance. The nature of light, and its influence on the function of the microscope, as well as practical advice on selecting the most suitable components, is explained to the reader. An account of the different types of currently-available light microscopy modes follows, together with a guide, borne out by the authors practical experience, on how to choose between transmitted or reflected light microscopes; wide-field, confocal, deconvolution or multi-photon microscopes. A description of specimen preparation is given, as well as advice upon cameras, image acquisition and post-processing of data. * Gives a didactic introduction to the light microscope * Explanation complemented with simple experiments where appropriate * Simple explanation (from teaching the subject) enables a self-taught student to start from scratch to set up and acquire high quality images * Leads the student on, should they wish, to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility * Well-illustrated in colour, the illustrations are selected (from long teaching experience) to support text * Workable practical protocols * Includes a chapter summary to introduce learning objectives Part of the Royal Microscopical Society co-publication book program

Understanding Light Microscopy

Spis treści

About the Author ix

Acknowledgements xi

Look-Up Guide to Feature Boxes by Theme xii

Glossary and Definitions xiv

Notes xxiv

Introduction xxvii

1 Our Eyes and the Microscope 1

2 Light 29

3 Basic Microscope Optics 55

4 Microscope Anatomy and Design 75

5 Ergonomics 91

6 Optical Aberrations of the Microscope 101

7 The Microscope Objective 127

8 Condensers and Eyepieces 161

9 Illumination in the Microscope 177

10 Diffraction and Image Formation in Microscopy 211

11 Contrast Generation and Enhancement 243

12 Reflected-Light Microscopy 289

13 Polarised-Light Microscopy: Part 1 - Theory 317

14 Polarised-Light Microscopy: Part 2 - Applied 347

15 Fluorescence Microscopy 383

16 Fluorophores and Fluorescent Proteins 405

17 Optical Sectioning and Confocal Microscopy 425

18 Operating the Confocal Microscope 447

19 Light-Sheet Microscopy 483

20 Bleed-Through and Spectral Unmixing 507

21 Deconvolution 523

22 Multi-Photon Microscopy 543

23 Total Internal Reflection Fluorescence Microscopy 561

24 FRAP and FRET 569

25 Colocalisation 587

26 Super-Resolution Fluorescence Microscopy 613

27 Choosing a Microscope Platform and Core Imaging Facilities 637

28 Biological Specimen Preparation 663

29 Materials Specimen Preparation 687

30 Recording the Image: Part 1 - Theory 707

31 Recording the Image: Part 2 - Applied 733


1 Buying, and Tendering for, a Light Microscope 769

2 Troubleshooting Poor Image Quality 773

3 The Michel-Levy Interference Colour Chart 775

4 Cleaning and Maintenance of the Light Microscope 779

5 Selected Suppliers 783

6 Historical Background 787

7 Timeline of Key Events 791

Index 799

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