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Co- and Post-Translational Modifications of Therapeutic Antibodies and Proteins

Co- and Post-Translational Modifications of Therapeutic Antibodies and Proteins

Autorzy
Wydawnictwo Blackwell Science
Data wydania 01/03/2019
Wydanie Pierwsze
Liczba stron 304
Forma publikacji książka w twardej oprawie
Poziom zaawansowania Dla profesjonalistów, specjalistów i badaczy naukowych
Język angielski
ISBN 9781119053316
Kategorie Biologia
614.00 PLN (z VAT)
$162.22 / €134.83 / £116.47 /
Produkt dostępny
Dostawa 2 dni
Ilość
Do schowka

Opis książki

A Comprehensive Guide to Crucial Attributes of Therapeutic Proteins in Biological Pharmaceuticals With this book, Dr. Raju offers a valuable resource for professionals involved in research and development of biopharmaceutical and biosimilar drugs. This is a highly relevant work, as medical practitioners have increasingly turned to biopharmaceutical medicines in their search for safe and reliable treatments for complex diseases, while pharmaceutical researchers seek to expand the availability of biopharmaceuticals and create more affordable biosimilar alternatives. Readers receive a thorough overview of the major co-translational modifications (CTMs) and post-translational modifications (PTMs) of therapeutic proteins relevant to the development of biotherapeutics. The majority of chapters detail individual CTMs and PTMs that may affect the physicochemical, biochemical, biological, pharmacokinetic, immunological, toxicological etc. properties of proteins. In addition, readers are guided on the methodology necessary to analyze and characterize these modifications. Thus, readers gain not only an understanding of CTMs/PTMs, but also the ability to design and assess their own structure-function studies for experimental molecules. Specific features and topics include: Discussion of the research behind and expansion of biopharmaceuticals Twenty chapters detailing relevant CTMs and PTMs of proteins, such as glycosylation, oxidation, phosphorylation, methylation, proteolysis, etc. Each chapter offers an introduction and guide to the mechanisms and biological significance of an individual CTM or PTM, including practical guidance for experiment design and analysis An appendix of biologic pharmaceuticals currently on the market, along with an assessment of their PTMs and overall safety and efficacy This volume will prove a key reference on the shelves of industry and academic researchers involved in the study and development of biochemistry, molecular biology, biopharmaceuticals and proteins in medicine, particularly as biopharmaceuticals and biosimilars become ever more prominent tools in the field of healthcare.

Co- and Post-Translational Modifications of Therapeutic Antibodies and Proteins

Spis treści

Preface xv


About the Author xix


Abbreviations xxi


1 Introduction to Co- and Post-translational Modifications of Proteins 1


Brief Introductions to Individual Chapters 8


Chapter 2: Acetylation of Proteins 8


Chapter 3: C-Terminal Lys or Arg Clipping of Proteins 8


Chapter 4: Cysteinylation of Proteins 8


Chapter 5: Deamidation of Proteins 8


Chapter 6: Glycation of Proteins 9


Chapter 7: Glycosylation of Proteins 9


Chapter 8: N-glycosylation of Proteins 9


Chapter 9: O-glycosylation of Proteins 10


Chapter 10: Hydroxylation of Proteins 10


Chapter 11: Methylation of Proteins 10


Chapter 12: Oxidation of Proteins 11


Chapter 13: Phosphorylation of Proteins 11


Chapter 14: Prenylation of Proteins 11


Chapter 15: Proteolysis of Proteins 11


Chapter 16: Selenylation of Proteins 12


Chapter 17: Signal Peptides of Proteins 12


Chapter 18: Sulfation of Proteins and Glycoproteins 12


Chapter 19: SUMOylation 12


Chapter 20: Ubiquitination 13


Chapter 21: Other PTMs 13


References 13


2 Acetylation of Proteins 17


Introduction 17


Mechanism of N-acetylation at the N-termini of Proteins 19


Mechanism of N-acetylation and N-deacetylation of Lysine Residues 21


Mechanism of O-acetylation of Sugar Residues 21


Biological Significance of Protein Acetylation 22


Acetylation in Recombinant Therapeutic Proteins 23


Methods to Analyze Acetylation in Proteins and Carbohydrates 23


References 24


3 C-terminal Lys or Arg Clipping in Proteins 31


Introduction 31


Biological Significance of C-terminal Lys or Arg Clipping in Proteins 31


Analysis of C-terminal Lys or Arg Clipping in Proteins 32


References 32


4 Cysteinylation of Proteins 35


Introduction 35


Biological Significance of Cysteinylation of Proteins 35


Cysteinylation and Trisulfide Bonds in Recombinant Therapeutic Proteins 36


Analysis of Cysteinylation of Proteins 36


References 37


5 Deamidation of Proteins 39


Introduction 39


Mechanism of Deamidation of Proteins 40


Physicochemical Characteristics of Deamidated Proteins 42


Biological Significance of Deamidation of Proteins 43


Deamidation and Immunogenicity 43


Deamidation and Pharmacokinetics Properties of Proteins 44


Deamidation in Recombinant Therapeutic Proteins 44


Methods for the Analysis of Deamidation in Proteins 44


References 45


6 Glycation of Proteins 51


Introduction 51


Mechanism of Protein Glycation 52


Glycation of Proteins in Human 54


Protein Glycation and Human Diseases 55


Glycation in Recombinant Therapeutic Proteins 56


Methods to A

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