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Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals.

Coming Soon in the Fungal Biology series:

Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens

Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites

Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2

van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi

Schmoll, Dattenbock / Gene Expression Systems in Fungi

Dahms / Advanced Microscopy in Mycology

Laboratory Protocols in Fungal Biology: Current Methods in Fungal Biology

1. Safety Norms and Regulations in Handling Fungal Specimens

Finola E. Cliffe

2. Methods of Cryopreservation in Fungi

Ladislav Homolka

3. Long Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM): Protocols and Results

Svetlana M. Ozerskaya, Natalya E. Ivanushkina, Galina A. Kochkina, Svetlana S. Eremina, Alexander N. Vasilenko, and Nadezhda I. Chigineva

4. Fungal Specimen Collection and Processing.

Anthonia O'Donovan, Vijai Kumar Gupta, and Maria G. Tuohy

5. Chemical and Molecular Methods for Detection of Toxigenic Fungi and Their Mycotoxins from Major Food Crops

S. Chandra Nayaka, A.C. Udayashankar, M.Venkata Ramana, S.R. Niranjana, C. N. Mortensen, and H.S. Prakash

6. Identification Key for the Major Growth Forms of Lichenized Fungi

Jayabalan Sangeetha and Devarajan Thangadurai

7. Microscopic Methods for Analytical Studies of Fungi

De-Wei Li

8. Scanning Electron Microscopy for Fungal Sample Examination

Eduardo Alves, Gilvaine Ciavareli Lucas, Edson Ampélio Pozza, and Marcelo de Carvalho Alves

9. High Resolution Imaging and Force Spectroscopy of Fungal Hyphal Cells by Atomic Force Microscopy

Tanya Dahms

10. Use of Fourier-Transform Infrared (FTIR) Microscopy Method For Detection of Phyto-Fungal Pathogens

Vitaly Erukhimovitch and Mahmoud Huleihel

11. Diagnose of Parasitic Fungi in The Plankton: Technique for Identifying And Counting Infective Chytrids Using Epifluorescence Microscopy

Télesphore Sime-Ngando, Serena Rasconi, and Mélanie Gerphagnon

12. Fungal Cell Wall Analysis

Pilar Pérez and Juan C. Ribas

13. Histopathological Technique for Detection of Fungal Infections in Plants

Vijai Kumar Gupta and Brejesh Kumar Pandey

14. Development of Media for Growth and Enumeration of Fungi from Water

Segula Masaphy

15. Sabouraud Agar for Fungal Growth

Janelle M. Hare

16. A Method for the Formation of Candida Biofilms in 96 Well Microtiter Plates and its Application to Antifungal Susceptibility Testing

Christopher G. Pierce, Priya Uppuluri, and Jose L. Lopez-Ribot

17. Screening for Compounds Exerting Antifungal Activities

Jean-Paul Ouedraogo, Ellen L. Lagendijk, Cees A.M.J.J van den Hondel, Arthur F.J. Ram, and Vera Meyer

18. Fluorescence In situ Hybridization of Uncultured Zoosporic Fungi

Télesphore Sime-Ngando, Marlene Jobard, and Serena Rasconi

19. Staining Techniques and Biochemical Methods for the Identification of Fungi

Jayabalan Sangeetha and Devarajan Thangadurai

20. Protocol for the In vivo Quantification of Superoxide Radical in Fungi

Grintzalis Konstantinos, Papapostolou Ioannis, and Christos Georgiou

21. Isolation of Intact RNA from Sorted S. cerevisiae Cells for Differential Gene Expression Analysis

Jeannette Vogt, Frank Stahl, Thomas Scheper, and Susann Müller

22. Quantitative PCR Analysis of Double-Stranded RNA-Mediated Gene Silencing in Fungi

José Javiar de Vega-Bartol, María Vega Tello Hernández, Jonathan Nino Sánchez, Virginia Casado del Castillo, and José M. Díaz-Mínguez

23. Semi-nested PCR Approach to Amplify Large 8S rRNA Gene Fragments for PCR-DGGE Analysis of Soil Fungal Communities

Miruna Oros-Sichler and Kornelia Smalla

24. Proteomic Protocols for the Study of Filamentous Fungi

Raquel González Fernández and Jesús V. Jorrín Novo

25. Detection and Quantification of Endoprotease Activity Using a Coomassie Dye-Binding Assay

Anthony J. O'Donoghue and Cathal S. Mahon

26. Protocol of a LightCyclerÔ PCR Assay for Detection and Quantification of Aspergillus fumigatus DNA in Clinical Samples of Neutropenic Patients

Birgit Spiess and Dieter Buchheidt

27. Application of Polymerase Chain Reaction (PCR) and PCR Based Methods Targeting Internal Transcribed Spacer (ITS) for Detection and Species Level Identification of Fungi

K. Lily Therese, R. Bagyalakshmi, and H. N. Madhavan

28. Real-Time PCR Assay in Fungi

Naomichi Yamamoto

29. Quantitative Sampling Methods for the Analysis of Fungi: Air Sampling

Mary C. O'Loughlin, Katherine D. Turner, and Kevin M. Turner

30. Transformation of Filamentous Fungi in Microtiter Plate

Bianca Gielesen and Marco van den Berg

31. Molecular Fingerprinting of Fungal Communities in Soil

Roberto A. Geremia and Lucie Zinger

32. Development of Microsatellite Markers from Fungal DNA Based on Shotgun Pyrosequencing

Shaobin Zhong

33. Multiplex and Quantifiable Detection of Infectious Fungi Using Padlock Probes, General qPCR and Suspension Microarray Readout

Magnus Jobs, Ronnie Eriksson, and Jonas Blomberg

34. Rapid Deletion Plasmid Construction Methods for Protoplast and Agrobacterium-based Fungal Transformation Systems

María D. García-Pedrajas, Zahi Paz, David L. Andrews, Lourdes Baeza-Montanez, and Scott E. Gold

35. Improved Transformation Method for Alternaria brassicicola and its Applications

Yangrae Cho, Akhil Srivastava, and Christopher Nguyen

36. Methods for High Quality DNA Extraction from Fungi

Vijai Kumar Gupta, Maria G. Tuohy and Rajeeva Gaur

37. Production of Recombinant Proteins from Pichia pastoris: Interfacing Fermentation and Immobilized Metal Ion Affinity Chromatography

Berend Tolner, Gaurav Bhavsar, Bride Foster, Kim Vigor, and Kerry Chester

38. Development of a Real-Time Quantitative PCR Assay for the Assessment of Uncultured Zoosporic Fungi

Télesphore Sime-Ngando and Marlene Jobard

39. Nucleic and Protein Extraction Methods for Fungal Exopolysaccharide Producers

Jochen Schmid, Dirk Mueller-Hagen, Volker Sieber, and Vera Meyer

40. Directed Evolution of A Fungal Xylanase for Improvement of Thermal and Alkaline Stability

Dawn Elizabeth Stephens, Suren Singh, and Kugen Permaul

41. Genome Shuffling Protocol for the Pentose-Fermenting Yeast Scheffersomyces stipitis

Paramjit K. Bajwa, Nicole K. Harner, Terri L. Richardson, Sukhdeep Sidhu, Marc B. Habash, Jack T. Trevors, and Hung Lee

42. Detection and Identification of Fungal Microbial Volatile Organic Compounds by HS-SPME-GC-MS

Bernhard Kluger, Susanne Zeilinger, Gerlinde Wiesenberger, Denise Schöfbeck, and Rainer Schuhmacher

43. Transformation Methods for Slow Growing Fungi

Suman Mukherjee and Rebecca Creamer

44. Enzymatic Saccharification of lignocellulosic biomass

Manimaran Ayyachamy, Vijai Kumar Gupta, Finola E. Cliffe, and Maria G. Tuohy

45. Protoplast Fusion Techniques in Fungi

Annie Juliet Gnanam

46. Large Scale Production of Lignocellulolytic Enzymes in Thermophilic Fungi

Manimaran Ayyachamy, Mary Shier, and Maria G. Tuohy

47. Panfungal PCR Method for Detection of Aflatoxigenic Molds

Malik M. Ahmad, Pravej Alam, M. Z. Abdin, and Saleem Javed

48. Protocols for the Quantification of dsDNA and Its Fragmentation Status in Fungi

Ioannis Papapostolou, Konstantinos Grintzalis, and Christos Georgiou

49. Rapid Identification and Detection of Pathogenic Fungi by Padlock Probes

Clement K. M. Tsui, Bin Wang, Cor D. Schoen, and Richard C. Hamelin

50. Drug-Induced Permeabilization in Fungi

Maria D. Mayan, Alexandra MacAleenan, and Priscilla Braglia

51. Extraction and Characterization of Taxol: An Anticancer Drug from an Endophytic And Pathogenic Fungi

M. Pandi P. Rajapriya and P. T. Manoharan

52. Identification of Mycotoxigenic Fungi using an Oligonucleotide Microarray

Eugenia Barros

53. DNA Microarray-Based Detection and Identification of Fungal Specimens

Minna Mäki

54. Bioinformatic Protocols and the Knowledge-Base for Secretomes in Fungi

Gengkon Lum and Xiang Jia Min

55. High-Throughput Functional Annotation and Data Mining of Fungal Genomes to Identify Therapeutic Targets

Gagan Garg and Shoba Ranganathan

56. Application of Support Vector Machines in Fungal Genome and Proteome Annotation

Sonal Modak, Shimantika Sharma, Prashant Prabhakar, Akshay Yadav, and V. K. Jayaraman

57. Bioinformatics Tools for the Multilocus Phylogenetic Analysis of Fungi

Devarajan Thangadurai and Jayabalan Sangeetha