| Wydawnictwo | Springer, Berlin |
| Data wydania | |
| Liczba stron | 375 |
| Forma publikacji | książka w twardej oprawie |
| Język | angielski |
| ISBN | 9781493973088 |
| Kategorie | Mikrobiologia (nie medyczna) |
This detailed volume provides scientists interested in quorum sensing with a broad spectrum of methods and protocols useful for studying bacterial communication processes at the chemico-physical, molecular, and physiological level. Divided into three sections, the content covers detection and quantification of quorum sensing signal molecules, methods for the studying of quorum sensing at the molecular, physiological, and population level, as well as identification and characterization of anti-quorum sensing agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Quorum Sensing: Methods and Protocols serves as a comprehensive guide to the most important methodology currently available in the field.
Quorum Sensing: Methods and Protocols
Part I: Detection and Quantification of Quorum Sensing Signal Molecules
1. Use of Whole Cell Bioassays for Screening Quorum Signaling, Quorum-Interference, and Biofilm Dispersion
Starla G. Thornhill and Robert J.C. McLean
2. Detection of 2-Alkyl-4-Quinolones Using Biosensors
Matthew P. Fletcher, Stephen P. Diggle, Miguel Cámara, and Paul Williams
3. "Hot Stuff": The Many Uses of a Radiolabel Assay in Detecting Acyl-Homoserine Lactone Quorum Sensing Signals
Amy L. Schaefer, Caroline S. Harwood, and E. Peter Greenberg
4. Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of -Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs)
François Lépine, Sylvain Milot, Marie-Christine Groleau, and Eric Déziel
5. Detection of the Bacterial Quorum Sensing Signaling Molecules N-Acyl Homoserine Lactones (HSL) and N-Acyl-Homoserine (HS) with an Enzyme-Linked Immunosorbent Assay (ELISA) and via Ultrahigh Performance Liquid Chromatography Coupled to Mass Spectrometry (UHPLC-MS)
Michael Rothballer, Jenny Uhl, Josie Kunze, Philippe Schmitt-Kopplin, and Anton Hartmann
6. Biosensors for the Detection and Quantification of AI-2 Class Quorum Sensing Compounds
Sathish Rajamani and Richard Sayre
7. Detection of Agr-Type Autoinducing Peptides Produced by Staphylococcus aureus
Ewan J. Murray and Paul Williams
8. Ultra Performance Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of Diffusible Signal Factor (DSF)-Family Quorum Sensing Signals
Lian Zhou, Shuang Sun, Wei Zhang, and Ya-Wen He
9. Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode
Alyah Buzid, John H.T. Luong, F. Jerry Reen, Fergal O'Gara, Jeremy D. Glennon, and Gerard P. McGlacken
10. Detection and Quantification of Butyrolactones from Streptomyces
Marc Biarnes-Carrera, Rainer Breitling, and Eriko Takano
Part II: Methods for the Studying of Quorum Sensing at the Molecular, Physiological, and Population Level
11. Fluorescence Quenching Studies of Gamma-Butyrolactone Binding Protein (CprB) from Streptomyces coelicolor A3(2)
Jessy Mariam and Ruchi Anand
12. Methods to Study Solo/Orphan Quorum Sensing Receptors
Vittorio Venturi, Sujatha Subramoni, Anice Sabag-Daigle, and Brian M.M. Ahmer
13. Enzymatic Assays to Investigate Acyl-Homoserine Lactone Autoinducer Synthases
Daniel Shin and Rajesh Nagarajan
14. Global Expression Analysis of Quorum Sensing-Controlled Genes by RNAseq
Charlotte D. Majerczyk
15. Identification of AHL- and BDSF-Controlled Proteins in Burkholderia cenocepacia by Proteomics
Yilei Liu, Gabriella Pessi, Katharina Riedel, and Leo Eberl
16. Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo
Louise Dahl Hultqvist, Maria Alhede, Tim Holm Jakobsen, Michael Givskov, and Thomas Bjarnsholt
17. Assessing Pseudomonas aeruginosa Autoinducer Effects on Mammalian Epithelial Cells
Jake Everett, Rebecca Gabrilska, Kendra Rumbaugh, and Elena Vikström
18. Animal Models for Pseudomonas aeruginosa Quorum Sensing Studies
Damien Maura, Arunava Bandyopadhaya, and Laurence G. Rahme
19. Methods to Study Quorum Sensing-Dependent Virulence and Movement of Phytopathogens In Planta
Shulamit Manulis-Sasson and Laura Chalupowicz
20. Differential Equations Models to Study Quorum Sensing
Judith Pérez-Velázquez and Burkhard A. Hense
Part III: Identification and Characterization of Anti-Quorum Sensing Agents
21. Qualitative and Quantitative Determination of Quorum Sensing Inhibition In Vitro
Tim Holm Jakobsen, Maria Alhede, Louise Dahl Hultqvist, Thomas Bjarnsholt, and Michael Givskov
22. A Co-Culture-Based Approach for Screening Campaigns Aimed at Identifying Novel Pseudomonas aeruginosa Quorum Sensing Inhibitors
Giordano Rampioni, Giulia Giallonardi, Francesca D'Angelo, and Livia Leoni
23. A Culture-Dependent Method for the Identification of Quorum Quenching Enzymes of Microbial Origin
Kaihao Tang and Xiao-Hua Zhang
24. Directed Evolution of Quorum Quenching Enzymes: A Method for the Construction of a Directed Evolution Platform and Characterization of a Quorum-Quenching Lactonase from Geobacillus kaustophilus
Maybelle Kho Go, Jeng Yeong Chow, and Wen Shan Yew
25. Generation of High-Sensitivity Monoclonal Antibodies Specific for Homoserine Lactones
Soumya Palliyil
26. Identification of AI-2 Quorum Sensing Inhibitors in Vibrio harveyi through Structure-Based Virtual Screening
Tianyu Jiang, Peng Zhu, Lupei Du, and Minyong Li
27. Identification of Staphylococcal Quorum Sensing Inhibitors by Quantification of o-Hemolysin with High Performance Liquid Chromatography
Cassandra L. Quave and Alexander R. Horswill
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