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This third edition provides a wide range of different technologies, ranging from conventional growth basic techniques, application of molecular biology, development of resistance mutations, and diagnosis and monitoring treatment response. New and updated chapters cover techniques from the microscopic scale to whole animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Antibiotic Resistance Protocols, Third Edition aims to ensure successful results in the further study of this vital field.

Antibiotic Resistance Protocols

1. Methods for Measuring the Production of Quorum Sensing Signal Molecules

Manuel Alcalde-Rico and José Luis Martínez

2. Construction and Use of Staphylococcus aureus Strains to Study Within-Host Infection Dynamics

Gareth McVicker, Tomasz K. Prajsnar, and Simon J. Foster

3. Method for Detecting and Studying Genome-wide Mutations in Single Living Cells in Real-time

Marina Elez, Lydia Robert, and Ivan Matic

4. Detecting Phenotypically Resistant Mycobacterium tuberculosis using Wavelength Modulated Raman Spectroscopy

Vincent O. Baron, Mingzhou Chen, Simon O. Clark, Ann Williams, and Stephen H. Gillespie

5. A Flow Cytometry Method for Assessing M. tuberculosis Responses to Antibiotics Charlotte L. Hendon-Dunn, Stephen R. Thomas, Stephen C. Taylor, and Joanna Bacon

6. Application of Continuous Culture for Assessing Antibiotic Activity against Mycobacterium tuberculosis

Charlotte Hendon-Dunn, Saba Anwar, Christopher Burton, and Joanna Bacon

7. Real-Time Digital Bright Field Technology for Rapid Antibiotic Susceptibility Testing Chiara Canali, Erik Spillum, Martin Valvik, Niels Agersnap, and Tom Olesen

8. Enhanced Methodologies for Detecting Phenotypic Resistance in Mycobacteria

Robert J H Hammond, Vincent Baron, Sam Lipworth, and Stephen H. Gillespie

9. Methods to Determine Mutational Trajectories after Experimental Evolution of Antibiotic Resistance

Douglas Huseby and Diarmaid Hughes

10. Selection of ESBL-producing E. coli in a Mouse Intestinal Colonization Model

Frederik Boëtius Hertz, Karen Leth Nielsen, and Niels Frimodt-Moller

11. Transcriptional Profiling Mycobacterium tuberculosis from Patient Sputa

Leticia Muraro-Wildner, Katherine A Gould, and Simon J Waddell

12. Direct Agarose Gel Probing, Pulsed Field Gel Electrophoresis, Plasmid detection, Genomic Location, and Resistance Gene Mapping

Mark A Toleman

13. Using RT qPCR for Quantifying Mycobacteria marinum from In-vitro and In-vivo Samples

Han Xaio and Stephen H. Gillespie

14. Use of Larval Zebrafish Model to Study Within-Host Infection Dynamics

Tomasz K. Prajsnar, Gareth McVicker, Alexander Williams, Stephen A. Renshaw, and Simon J. Foster

Yanmin Hu and Anthony Coates