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Microbial Metabolomics

Microbial Metabolomics

Wydawnictwo Humana Press
Data wydania 01/11/2018
Wydanie Pierwsze
Liczba stron 351
Forma publikacji książka w twardej oprawie
Poziom zaawansowania Dla profesjonalistów, specjalistów i badaczy naukowych
Język angielski
ISBN 9781493987566
Kategorie Mikrobiologia (nie medyczna)
683.00 546.40 PLN
(z VAT)
$137.90 / €127.08 / £106.12 /
Rabat: 20%
Produkt dostępny
Dostawa 2 dni
Do schowka

Opis książki

This detailed volume includes protocols that represent the breadth of microbial metabolomics approaches to both large-scale and small-scale experiments with intention of highlighting techniques that can be used for applications ranging from environmental microbiology to human disease. Utilizing mass spectrometry as their primary measurement tool, the chapters explore microbial metabolomics, metabolism and microbial physiology, metabolite sample preparation, current analytical techniques used to profile primary and secondary metabolites and lipids, as well as establishing data analysis workflows for targeted metabolomics, untargeted metabolomics, analysis of metabolic fluxes, and genome-scale models. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Metabolomics: Methods and Protocols serves as an ideal reference for both novice and advanced users and can be adapted to similar analytical platforms or customized to suit the needs of the researcher.

Microbial Metabolomics

Spis treści

1. Microbial Metabolomics: A General Overview

Edward E.K. Baidoo

Part I: Microbial Metabolomics, Metabolism, and Physiology

2. Mass Spectrometry-Based Microbial Metabolomics: Techniques, Analysis, and Applications

Edward E.K. Baidoo and Veronica Teixeira Benites

3. Metabolomics: A Microbial Physiology and Metabolism Perspective

Chijioke J. Joshua

Part II: Metabolite Sample Preparation

4. Untargeted Soil Metabolomics Using Liquid Chromatography-Mass Spectrometry and Gas Chromatography-Mass Spectrometry

Tami L. Swenson and Trent R. Northen

5. Fatty Acid Metabolome Extraction from Mycobacterial Cells for GC-MS Metabolomics Analysis

Ilse du Preez, Derylize Beukes, and Du Toit Loots

6. Total Metabolome Extraction from Mycobacterial Cells for GC-MS Metabolomics Analysis

Derylize Beukes, Ilse du Preez, and Du Toit Loots

7. High-Throughput Solid Phase Microextraction-Liquid Chromatography-Mass Spectrometry for Microbial Untargeted Metabolomics

Fatemeh Mousavi, Barbara Bojko, and Janusz Pawliszyn

Part III: Current Analytical Techniques

8. Targeted Metabolomics of Xylose Fermenting Yeasts Based on Mass Spectrometry

Christiane Goncalves Campos, Jose Antonio de Aquino Ribeiro, Joao Ricardo Moreira de Almeida, Betania Ferraz Quirino, and Patricia Verardi Abdelnur

9. Exploiting High-Resolution Mass Spectrometry for Targeted Metabolite Quantification and 13C-labeling Metabolism Analysis

Zhucui Li, Yujing Li, Yinjie J. Tang, and Wenqing Shui

10. Quantitative Profiling of Endogenous Metabolites Using Hydrophilic Inter-Action Liquid Chromatography-Tandem Mass Spectrometry (HILIC-MS/MS)

Attila Teleki and Ralf Takors

11. Liquid Chromatography and Mass Spectrometry Analysis of Isoprenoid Intermediates in Escherichia coli

Edward E.K. Baidoo, George Wang, Chijioke Joshua, Veronica Teixeira Benites, and Jay D. Keasling

12. Determining the Mode of Action of Antimalarial Drugs Using Time-Resolved LC-MS-Based Metabolite Profiling

Simon A. Cobbold and Malcolm J. McConville

13. Use of Liquid Chromatography-Mass Spectrometry-Based Metabolomics to Identify Biomarkers of Tuberculosis

Juntuo Zhou and Yuxin Yin

14. Metabolomics Analysis of Leishmania by Capillary Electrophoresis and Mass Spectrometry

David Rojo, Coral Barbas, and Angeles Lopez-Gonzalvez

Part IV: Data Analysis

15. A High

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