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Plant Long Non-Coding RNAs: Methods and Protocols

Plant Long Non-Coding RNAs: Methods and Protocols

Wydawnictwo Humana Press Inc.
Data wydania 03/04/2019
Liczba stron 480
Forma publikacji książka w twardej oprawie
Poziom zaawansowania Dla profesjonalistów, specjalistów i badaczy naukowych
ISBN 9781493990443
Kategorie Genetyka (nie medyczna), Biologia molekularna, Fizjologia roślin
762.00 PLN (z VAT)
$202.74 / €170.31 / £151.73 /
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Opis książki

This volume focuses on various approaches to studying long non-coding RNAs (lncRNAs), including techniques for finding lncRNAs, localization, and observing their functions. The chapters in this book cover how to catalog lncRNAs in various plant species; determining subcellular localization; protein interactions; structures; and RNA modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and innovative, Plant Long Non-Coding RNAs: Methods and Protocols is a valuable resource that aids researchers in understanding the functions of lncRNAs in different plant species, and helps them explore currently uncharted facets of plant biology.

Plant Long Non-Coding RNAs: Methods and Protocols

Spis treści

Table of Contents...
Contributing Authors...

1 An Overview of Methodologies in Studying lncRNAs in the High-Throughput Era: When Acronyms ATTACK!
Hsiao-Lin V. Wang and Julia A. Chekanova

Part I Role of ncRNAs in Regulation of Gene Expression and Development in Plants

2 Analysis of siRNA Precursors Generated by RNA Polymerase IV and RNA-Dependent RNA Polymerase 2 in Arabidopsis
Todd Blevins, Ram Podicheti, and Craig S. Pikaard

3 Identification of Long Non-Coding RNAs in the Developing Endosperm of Maize
Eundeok Kim, Yuqing Xiong, Byung-Ho Kang, and Sibum Sung

4 RNA Isolation and Analysis of lncRNAs from Gametophytes of Maize
Linqian Han, Lin Li, Gary J. Muehlbauer, John E. Fowler, and Matthew M. S. Evans

Part II Studying the Tissue and Cell-Type Specific lncRNAs

5 Improved Method of RNA Isolation from Laser Capture Microdissection (LCM) Derived-Plant Tissues
Vibhav Gautam, Archita Singh, Sharmila Singh, Swati Verma, and Ananda K. Sarkar

6 Medium Throughput RNA In Situ Hybridization of Serial Sections from Paraffin-Embedded Tissue Microarrays
Edith Francoz, Philippe Ranocha, Christophe Dun, and Vincent Burlat

7 Purification and Functional Analysis of Plant Long Non-Coding RNAs (lncRNA)
Trung Do, Zhipeng Qu, and Iain Searle

Part III Studying lncRNAs Associated with Response to Abiotic and Biotic Stresses in Plants

8 The Involvement of Long Non-Coding RNAs in Response to Plant Stress
Akihiro Matsui and Motoaki Seki

9 Subcellular Localization and Functions of Plant lncRNAs in Drought and Salt Stress Tolerance
Tao Qin and Liming Xiong

10 Discovery, Identification, and Functional Characterization of Plant Long Intergenic Non-Coding RNAs after Virus Infection
Ruimin Gao, Peng Liu, Nadia Irwanto, De Rong Loh, and Sek-Man Wong

Part IV Identification and Functional Analysis of lncRNAs

11 Bioinformatics Approaches to Studying Plant Long Non-Coding RNAs (lncRNAs): Identification and Functional Interpretation of lncRNAs from RNA-seq Data Sets
Hai-Xi Sun and Nam-Hai Chua

12 Identification of Novel lincRNA and Co-Expression Network Analysis using RNA Sequencing Data in Plants
Song Qi, Shamima Akter, and Song Li

13 An Easy-to-Follow Pipeline for Long Non-Coding RNA Identification: A Case Study in Diploid Strawberry Fragaria vesca
Chunying Kang and Zhongchi Liu

14 Reference-Based Identification of Long Non-Coding RNAs in Plants with Strand-Specific RNA-Sequencing Data
Xiao Lin, Meng Ni, Zhixia Xiao, Ting-Fung Chan, and Hon-Ming Lam

15 NAMS: Non-Coding Assessment in Magnoliophyta Species
Gaurav Sablok, Kun Sun, and Hao Sun

16 De novo Plant Transcriptome Assembly and Annotation using Illumina RNA-seq Reads
Stephanie C. Kerr, Federico Gaiti, and Milos Tanurdzic

Part V Purification of lncRNAs and Ribonucleoprotein Complexes, RNA Secondary Structure, and RNA Modifications

17 Identification of Long Non-Coding RNA-Protein Interactions through In Vitro RNA Pull-Down Assay with Plant Nuclear Extracts
Jun Sung Seo and Nam-Hai Chua

18 Analysis of Interaction between Long Non-Coding RNAs and Protein by RNA-Immunoprecipitation in Arabidopsis
Jun Sung Seo and Nam-Hai Chua

19 Trimolecular Fluorescence Complementation (TriFC) Assay for Visualization of RNA-Protein Interaction in Plants
Jun Sung Seo and Nam-Hai Chua

20 In Vivo Genome-Wide RNA Structure Probing with Structure-seq2
Laura E. Ritchey, Zhao Su, Sarah M. Assmann, and Philip C. Bevilacqua

21 Using Protein Interaction Profile Sequencing (PIP-seq) to Identify RNA Secondary Structure and RNA-Protein Interaction Sites of Long Non-Coding RNAs in Plants
Marianne C. Kramer and Brian D. Gregory

22 Computationally Characterizing Protein Bound Long Non-Coding RNAs and their Secondary Structure using Protein Interaction Profile Sequencing (PIP-seq) in Plants
Mengge Shan, Zachary D. Anderson, and Brian D. Gregory

23 Stalking Structure in Plant Long Non-Coding RNAs
Karissa Y. Sanbonmatsu

24 Transcriptome-Wide Mapping 5-methylcytosine by m5C RNA Immunoprecipitation Followed by Deep Sequencing in Plant
Xiaofeng Gu and Zhe Liang

Part VI Databases of Plant lncRNAs and How to Use Them

25 A Walkthrough to the Use of GreeNC: The Plant lncRNA Database
Andreu Paytuvi, Walter Sanseverino, and Riccardo Aiese Cigliano

26 CANTATAdb 2.0: Expanding the Collection of Plant Long Non-Coding RNAs
Michal Wojciech Szczesniak, Oleksii Bryzghalov, Joanna Ciomborowska-Basheer, and Izabela Makalowska

27 Experimentally Validated Plant lncRNAs in EVLncRNAs Database
Bailing Zhou, Huiying Zhao, Jiafeng Yu, Chengang Guo, Xianghua Dou, Feng Song, Guodong Hu, Zanxia Cao, Yuanxu Qu, Yuedong Yang, Yaoqi Zhou, Jihua Wang

Part VII Long-Range Chromatin Interactions and Chromosome Conformation Capture

28 In Situ Hi-C for Plants: An Improved Method to Detect Long-Range Chromatin Interactions
Sudharsan Padmarasu, Axel Himmelbach, Martin Mascher, and Nils Stein

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