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The past decade has witnessed the discovery of many genes with profound effects on bone. In Bone Research Protocols, recognized authorities with extensive hands-on experience present a wide-ranging catalog of laboratory techniques that will help researchers to investigate the mechanisms by which these genes affect bone mass and strength. Described in step-by-step detail, these readily reproducible methods cover such topics as the isolation and culture of bone cells, the preparation of bone tissue for histological and ultrastructural analysis, methods for the measurement of bone strength and for mechanical studies, and how to use digital imaging techniques in the analysis of bone. Though in vitro methods receive central attention, there are also protocols for in vivo analyses of bone formation and resorption.
State-of-the-art and highly practical, Bone Research Protocols offers both beginning and advanced laboratory researchers a major compendium of well-established techniques for studying bone cell function and metabolism.

Bone Research Protocols

"Part I. Culture of Osteoblasts and Osteocytes

Human Osteoblast Culture
James A. Gallagher

Osteoblast Isolation from Murine Calvariae and Long Bones
Astrid Bakker and Jenneke Klein-Nulend

Mineralizing Fibroblast-Colony-Forming Assays
Andrew Scutt, Lynsey Reading, Nanette Scutt, and Karen Still

Osteocyte Isolation and Culture
Peter J. Nijweide, Arie van der Plas, Marcel J. Alblas, and Jenneke Klein-Nulend

Part II. Culture of Osteoclasts. Isolated Osteoclast Cultures
Astrid Hoebertz and Timothy R. Arnett

Primary Isolation and Culture of Chicken Osteoclasts
Patricia Collin-Osdoby, Fred Anderson, and Philip Osdoby

Isolation and Purification of Rabbit Osteoclasts
Fraser P. Coxon, Julie C. Frith, Helena L. Benford, and Michael J. Rogers

Generating Human Osteoclasts from Peripheral Blood
Afsie Sabokbar and Nicholas A. Athanasou

Generating Human Osteoclasts from Bone Marrow and Peripheral Blood
Adrienne M. Flanagan and Helen M. Massey

Generating Murine Osteoclasts from Bone Marrow
Naoyuki Takahashi, Nobuyuki Udagawa, Sakae Tanaka, and Tatsuo Suda

Osteoclast Formation in the Mouse Coculture Assay
Robert J. van 't Hof

RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 Cells
Patricia Collin-Osdoby, Xuefeng Yu, Hong Zheng, and Philip Osdoby

Part III. Bone Organ Culture

Analysis of Osteoclast Function in Mouse Calvarial Cultures
Michael J. Marshall and Marit N. Rowlands

Assessing Bone Formation Using Mouse Calvarial Organ Culture
I. Ross Garrett

Part IV. Histological and Ultrastructural Techniques

In Situ Hybridization and In Situ Reverse Transcription Polymerase Chain Reaction in Human Bone Sections
Andrew P. Mee and Judith A. Hoyland

Techniques for the Study of Apoptosis in Bone
Brendon S. Noble an"