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A comprehensive collection of optimized methods for dissecting the mechanisms that control epidermal growth factors (EGF) and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Epidermal Growth Factor: Methods and Protocols

A Historical Perspective of the EGF Receptor and Related Systems
Francis Edwin, Gregory J. Wiepz, Rakesh Singh, Chimera Rene Peet, Deepti Chaturvedi, Paul J. Bertics, and Tarun B. Patel

Purification and Assay of Kinase-Active EGF Receptor From Mammalian Cells by Immunoaffinity Chromatography
Gregory J. Wiepz, Arturo G. Guadaramma, David L. Fulgham, and Paul J. Bertics

Soluble Epidermal Growth Factor Receptor Acridinium-Linked Immunosorbent Assay
Jacqueline M. Lafky, Andre T. Baron, and Nita J. Maihle

Analysis of EGF Receptor Interactions With the a Subunit of the Stimulatory GTP Binding Protein of Adenylyl Cyclase, Gs
Deepti Chaturvedi, Francis Edwin, Hui Sun, and Tarun B. Patel

Regulator of Epidermal Growth Factor Signaling: Sprouty
Esther Sook Miin Wong and Graeme R. Guy

Dissecting the Epidermal Growth Factor Receptor Signal Transactivation Pathway
Oliver M. Fischer, Stefan Hart, and Axel Ullrich

A Sensitive Method to Monitor Ectodomain Shedding of Ligands of the Epidermal Growth Factor Receptor
Umut Sahin, Gisela Weskamp, Yufang Zheng, Valerie Chesneau, Keisuke Horiuchi, and Carl P. Blobel

In Vitro and In Vivo Assays of Monoubiquitination of Receptor Tyrosine Kinases
Menachem Katz, Yaron Mosesson, and Yosef Yarden

Assays to Monitor Degradation of the EGF Receptor
Mirko H. H. Schmidt and Ivan Dikic

Assessment of Degradation and Ubiquitination of CXCR4, a GPCR Regulated by EGFR Family Members
Adriano Marchese

Epithelial Cell Migration in Response to Epidermal Growth Factor
Reema Zeineldin and Laurie G. Hudson

Motility Signaled From the EGF Receptor and Related Systems
Alan Wells, Brian Harms, Akihiro Iwabu, Lily Koo, Kirsty Smith, Linda Griffith, and Douglas A. Lauffenburger

Methods for Determining the Proliferation of Cells in Response to EGFR Ligands
Gregory J. Wiepz, Francis Edwin, Tarun B. Patel, and Paul J. Bertics

Clinical Advancement of EGFR Inhibitors in Cancer Therapy
Prakash Chinnaiyan and Paul M. Harari

Index