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An Introduction to Molecular Biotechnology

An Introduction to Molecular Biotechnology

Autorzy
Wydawnictwo VCH
Data wydania 01/03/2011
Wydanie Drugie
Liczba stron 636
Forma publikacji książka w miękkiej oprawie
Poziom zaawansowania Dla profesjonalistów, specjalistów i badaczy naukowych
Język angielski
ISBN 9783527326372
Kategorie Biologia, Ewolucja
516.02 PLN (z VAT)
$136.83 / €115.92 / £105.58 /
Produkt na zamówienie
Dostawa 5-6 tygodni
Ilość
Do schowka

Opis książki

Molecular biotechnology continues to triumph, as this textbook testifies - edited by one of the academic pioneers in the field and written by experienced professionals. This completely revised second edition covers the entire spectrum, from the fundamentals of molecular and cell biology, via an overview of standard methods and technologies, the application of the various "-omics", and the development of novel drug targets, right up to the significance of system biology in biotechnology. The whole is rounded off by an introduction to industrial biotechnology as well as chapters on company foundation, patent law and marketing. The new edition features: - Large format and full color throughout - Proven structure according to basics, methods, main topics and economic perspectives - New sections on system biology, RNA interference, microscopic techniques, high throughput sequencing, laser applications, biocatalysis, current biomedical applications and drug approval - Optimized teaching with learning targets, a glossary containing around 800 entries, over 500 important abbreviations and further reading. The only resource for those who are seriously interested in the topic. Bonus material available online free of charge: www.wiley-vch.de/home/molecbiotech "Beautiful color illustrations make a complex subject easier to understand in this book on the fundamentals of molecular and cell biology... It is intended as a resource for those who are seriously interested in molecular biotechnology...Given the rapid advances in the area of molecular biotechnology, this updated second edition is an important resource for all those interested in this field." ( Doody's, 30 September 2011) "Overall, however, I am very satisfied with the contents of this book (I wish I could edit such a nice book !), and would like to recommend it to the undergraduate and graduate students alike who want to quickly yet thoroughly learn the fundamentals and many applications of molecular biotechnology. Also, this book will be a nice and smooth reading for professors, researchers, and professionals in the field of bioscience and biotechnology to update themselves who wish to catch up with the recent developments in molecular biotechnology. In conclusion, this book will be a must read for modern bioscientists and biotechnologists and those who want to become." (Chemical and Biomolecular Engineering, 1 May 2011) "This book is a must read for modern bioscientists and biotechnologists and those who want to become one. (Biotechnology Journal, 1 July 2011)

An Introduction to Molecular Biotechnology

Spis treści

Preface XIX List of Contributors XXI Abbreviations XXV Part I Fundamentals of Cellular and Molecular Biology 1 1 The Cell as the Basic Unit of Life 3 M. Wink 2 Structure and Function of Cellular Macromolecules 7 M. Wink 2.1 Structure and Function of Sugars 8 2.2 Structure of Membrane Lipids 10 2.3 Structure and Function of Proteins 14 2.4 Structure of Nucleotides and Nucleic Acids (DNA and RNA) 21 2.5 References 27 3 Structure and Functions of a Cell 29 M. Wink 3.1 Structure of a Eukaryotic Cell 29 3.2 Structure of Bacteria 50 3.3 Structure of Viruses 51 3.4 Differentiation of Cells 52 4 Biosynthesis and Function of Macromolecules (DNA, RNA, and Proteins) 57 M. Wink 4.1 Genomes, Chromosomes, and Replication 57 4.2 Transcription: From Gene to Protein 71 4.3 Protein Biosynthesis (Translation) 76 5 Distributing Proteins in the Cell (Protein Sorting) 81 M. Wink 5.1 Import and Export of Proteins via the Nuclear Pore 82 5.2 Import of Proteins in Mitochondria and Chloroplasts 83 5.3 Protein Transport into the Endoplasmic Reticulum 85 5.4 Vesicle Transport from the ER via the Golgi Apparatus to the Cytoplasmic Membrane 86 6 Evolution and Diversity of Organisms 91 M. Wink 6.1 Prokaryotes 91 6.2 Eukaryotes 91 Part II Standard Methods in Molecular Biotechnology 99 7 Isolation and Purification of Proteins 101 T. Wieland, M. Lutz 7.1 Introduction 101 7.2 Producing a Protein Extract 102 7.3 Gel Electrophoretic Separation Methods 103 7.4 Methods of Protein Precipitation 106 7.5 Column Chromatography Methods 107 7.6 Examples 113 8 Peptide and Protein Analysis with Electrospray Tandem Mass Spectrometry 115 A. Schlosser, W.D. Lehmann 8.1 Introduction 115 8.2 Principles of Mass Spectrometry 115 8.3 Mass Precision, Resolution, and Isotope Distribution 116 8.4 Principles of ESI 116 8.5 Tandem Mass Spectrometers 117 8.6 Peptide Sequencing with MS/MS 119 8.7 Identifying Proteins with MS/MS Data and Protein Databases 120 8.8 Determining Protein Molecular Mass 121 8.9 Analysis of Covalent Protein Modification 122 8.10 Relative and Absolute Quantification 123 9 Isolation of DNA and RNA 125 H. Weiher, R. Zwacka, I. Herr 9.1 Introduction 125 9.2 DNA Isolation 125 9.3 RNA Isolation 127 10 Chromatography and Electrophoresis of Nucleic Acids 129 H. Weiher, R. Zwacka, I. Herr 10.1 Introduction 129 10.2 Chromatographic Separation of Nucle

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