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This detailed volume explores numerous protocols that can be specifically used for studying plant programmed cell death (PCD), a mechanism involved in a number of physiological and pathological processes that are triggered by developmental requirements as well as changing/adverse environmental conditions. Rich in methodological recommendations, this book enables readers to perform the described analyses, alerting them to possible difficulties and showing how the methods can also be applied in different contexts and/or on different plant matrices. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Programmed Cell Death: Methods and Protocols provides essential methodological information that will help researchers to expand the field of plant PCD studies.

Plant Programmed Cell Death: Methods and Protocols

1. Programmed Cell Death in Plants: An Overview

Vittoria Locato and Laura De Gara

2. The Investigation of Morphological Features of Autophagy During Plant Programmed Cell Death

Alessio Papini

3. Markers of Developmentally-Regulated Programmed Cell Death and Their Analysis in Cereal Seeds

Fernando Domínguez and Francisco Javier Cejudo

4. Measurement of Hypersensitive Cell Death Triggered by Avirulent Bacterial Pathogens in Arabidopsis

Zahra Imanifard, Elodie Vandelle, and Diana Bellin

5. Immunity-Associated Programmed Cell Death as a Tool for the Identification of Genes Essential for Plant Innate Immunity

Bangjun Zhou and Lirong Zeng

6. Analysis of Mitochondrial Markers of Programmed Cell Death

Dong Xiao, Huyi He, Wenjing Huang, Thet Lwin Oo, Aiqin Wang, and Long-Fei He

7. Studying Retrograde Signaling in Plants

Martyna Broda and Olivier Van Aken

8. Reactive Oxygen Species and Cell Death Detection in Tomato Plants Infected by Root Knot Nematodes

Paola Leonetti

9. Detection of Reactive Oxygen and Nitrogen Species (ROS/RNS) During Hypersensitive Cell Death

Laura C. Terrón-Camero, Eliana Molina-Moya, María Sanz-Fernández, Luisa M. Sandalio, and María C. Romero-Puertas

10. DNA Diffusion Assay Applied to Plant Cells

Anca Macovei, Mattia Dona, Daniela Carbonera, and Alma Balestrazzi

11. Analysis of Reactive Carbonyl Species Generated Under Oxidative Stress

Jun'ichi Mano and Md. Sanaullah Biswas

12. In Vivo Analysis of Calcium Levels and Glutathione Redox Status in Arabidopsis Epidermal Leaf Cells Infected with the Hypersensitive Response-Inducing Bacteria Pseudomonas syringae pv. tomato AvrB (PstAvrB)

Fabrizio Gandolfo Doccula, Laura Luoni, Smrutisanjita Behera, Maria Cristina Bonza, and Alex Costa

13. Measurement of Cyclic GMP During Plant Hypersensitive Disease Resistance Response

Jian Chen, Diana Bellin, and Elodie Vandelle

14. Detection of MAPK3/6 Phosphorylation During Hypersensitive Response (HR)-Associated Programmed Cell Death in Plants

Qingyu Wu and David Jackson

15. Measurement of the Caspase-1-Like Activity of Vacuolar Processing Enzyme in Plants

Noriyuki Hatsugai and Ikuko Hara-Nishimura

16. Plant Cell Cultures as Model Systems to Study Programmed Cell Death

Cimini Sara, Ronci Maria Beatrice, Elisabetta Barizza, Maria Concetta de Pinto, Vittoria Locato, Fiorella Lo Schiavo, and Laura De Gara