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This cutting-edge book presents protocols and strategies for proteomic evaluation of cardiovascular disease written by pioneering researchers in the field. Topics explored in this comprehensive volume include obtaining specific heart proteins, techniques for identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, and phage display techniques for deciphering the molecular diversity of blood vessels.

Cardiovascular Proteomics: Methods and Protocols

Part I. Analysis of Heart Proteome

Two-dimensional Polyacrylamide Gel Electrophoresis for Cardiovascular Proteomics
Michael J. Dunn

Analyzing The Cardiac Muscle Proteome By Liquid Chromatography-Mass Spectrometry (Lc-ms) Based Expression Proteomics
Anthony O. Gramolini, Thomas Kislinger, Peter Liu, David H. Maclennan, and Andrew Emili

2-D Differential Gel Electrophoresis (DIGE) of Rat Heart Proteins in Ischemia and Ischemia-Reperfusion
Jun Sakai, Hironori Ishikawa, Hideshi Satoh, Setsuko Yamamoto, Shinichi Kojima, and Masaharu Kanoaka

Analysis of Antihypertensive Drugs in the Heart of Animal Models: A Proteomic Approach
Alberto Lázaro, Julio Gállego-delgado, Julio Osende, Jesús Egido, and Fernando Vivanco

A Solubility Optimization Protocol for 2-D Gel Electrophoresis of Cardiac Tissue
Brian A. Stanley and Jennifer E. Van Eyk

A Method for Effective Depletion of Albumin From Cellular Extracts: Application to Human Myocardium
Brian A. Stanley and Jennifer E. Van Eyk

Part II. Heart Subproteomes

Subcellular Fractionation
Mariana E. Guimaraes De Araújo and Alfons Huber

Subfractionation of Heart Tissue: The 'In Sequence' Myofilament Protein Extraction Of Myocardial Tissue
Lesley A. Kane, Irina Neverova, and Jennifer E. Van Eyk

Optimization of Cardiac Troponin I Pull-Down by Idm Affinity Beads and Seldi-tofT
Diane E. Bovenkamp, Brian A. Stanley and Jennifer E.van Eyk

Proteomic Analysis Of The Subunit Composition of Complex I (Nadh:Ubiquinone Oxidoreductase) From Bovine Heart Mitochondria
Ian M. Fearnley, Joe Carrol and John E. Walker

Identification of Targets of Phosphorylation in Heart Mitochondria
Roberta A. Gottliebie

Part III. Proteomics of Human Atheroma Plaques

Characterization of The Human Atheroma Plaque Secretome by Proteomic Analysis
Mari-CarmenDurán, Jose L. Martin-Ventura, Sebastián Mas, Maria G. Barderas, Verónica M. Dardé, Ole N. Jensen, Jesús Egido, and Fernando Vivanco

Characterization of Hsp-27 Phosphorylation Sites in Human Atherosclerotic Plaque Secretome
Mari-Carmen Durán, Elisabetta Boeri-Erba, Shabaz Mohammed, Jose L. Martín-Ventura, Jesús Egido, Fernando Vivanco, and Ole N. Jensen

Western Array Analysis of Human Atherosclerotic Plaque
Wim Martinetar

Part IV. The Proteome of Cells of The Cardiovascular System

The Proteome of Endothelial Cells
Jesús Gonzalez-Cabrero, Mayte Pozo, Mari-Carmen Durán, Rosario de Nicolás, Jesús Egido, and Fernando Vivanco

Proteomic Study of Caveolae and Rafts Isolated from Human Endothelial Cells
Richard R. Sprenger and Anton J. G. Horrevoets

Proteomic Identification of S-nitrosylated Proteins In Endothelial Cells
Antonio Martínez-Ruiz and Santiago Lamasgo

The Proteome and Secretome of Human Arterial Smooth Muscle Cell
Annabelle Dupont and Florence Pinet

Real-Time in Vivo Proteomic Identification Of Novel Kinase Substrates in Smooth Muscle
Anne A. Wooldridge and Timothy A. Haystead

Proteomic Analysis of Vascular Smooth Muscle Cells Treated With Ouabain
Alexy V. Pshezhetsky

Isolation and Culture of Adult Mouse Cardiac Myocytes
Timothy D. O´Connell, Manoj C. Rodrigo, and Paul C. Simpson

Proteomic Analysis Of Foam Cells
Peng-Yuang Yang, Yao-cheng Rui, Peng-Yuan Yang, and Yan-Ling Yuin

Isolation of The Platelet Releasate
Judith Coppinger, Desmond J. Fitzgerald, and Patricia B. Maguire

Enrichment Of Phospho-Tyrosine Proteome Of Human Platelets By Immunoprecipitation
Martina Foy, Donald F. Harney, Kieran Wynne, and Patricia B. Maguire

Chapter 25
Verónica M. Dardé, Mari-Carmen Durán, Jesús Egido, and Fernando Vivanco

Part V.