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The Handbook is intended to be a service to the neuroscience community, to help in finding available and useful information, to point out gaps in our knowledge, and to encourage continued studies. It represents the valuable contributions of the many authors of the chapters and the guidance of the editors and most important, it represents support for research in this discipline. Based on the rapid advances in the years since the second edition

Handbook of Neurochemistry and Molecular Neurobiology: Practical Neurochemistry Methods

GAS CHROMATOGRAPHY - K. A. Rittenbach and G. B. Baker.- HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF PSYCHOTROPIC AND ENDOGENOUS COMPOUNDS - J. Odontiadis and G. Rauw.- CYTOCHROME P450 REACTIONS IN THE HUMAN BRAIN - R. L. Haining.- PRACTICAL ENZYMOLOGY: QUANTIFYING ENZYME ACTIVITY AND THE EFFECTS OF DRUGS THEREUPON - A. Holt.- FLUORESCENCE MEASUREMENTS OF RECEPTOR-LIGAND INTERACTIONS - S. M. J. Dunn.- THE ANALYSES OF NEUROTRANSMITTERS, OTHER NEUROACTIVE SUBSTANCES, AND THEIR METABOLITES USING MASS SPECTROMETRY - B. D. Sloley, G. Rauw, and R. T. Coutts.- THE ANALYSIS OF NEUROACTIVE STEROIDS BY MASS SPECTROMETRY - R. H. Purdy, R. L. Fitzgerald, E. T. Everhart, S. H. Mellon, A. A. Alomary, and L. H. Parsons.- METHODS IN IMMUNOCHEMISTRY - L. L. Jantzie, V. A.-M. I. Tanay, and K. G. Todd.- IN VIVO MICRODIALYSIS: A METHOD FOR SAMPLING EXTRACELLULAR FLUID IN DISCRETE BRAIN REGIONS - D. L. Krebs-Kraft, K. J. Frantz, and M. B. Parent.- CHARACTERIZATIONS OF RECEPTORS BY RADIOLABELLED LIGAND BINDING TECHNIQUES - I. L. Martin, M. Davies, and S. M. J. Dunn.- ANALYSIS OF RECEPTOR LOCALIZATION IN THE CENTRAL NERVOUS SYSTEM USING IN VITRO AND IN VIVO RECEPTOR AUTORADIOGRAPHY - S. Kar and C. Hawkes.- IN SILICO MOLECULAR HOMOLOGY MODELING OF NEUROTRANSMITTER RECEPTORS - M. Wang, D. R. Hampson, and L. P. Kotra.- FLOW CYTOMETRY - W. P. Gati.- EXPRESSION AND STUDY OF LIGAND-GATED ION CHANNELS IN XENOPUS LAEVIS OOCYTES - A. Kapur, J. M. C. Derry, and R. S. Hansen.- NUCLEIC ACID QUANTITATION USING THE COMPETITIVE POLYMERASE CHAIN REACTION - J. Auta, Y. Chen, W. B. Ruzicka, and D. R. Grayson.- IN SITU HYBRIDIZATION - A. N. Bateson.- ANALYSIS OF GENE EXPRESSION IN THE BRAIN USING DIFFERENTIAL DISPLAY - K. L. Gilby and E. M. Denovan-Wright.- GENE ARRAYS: A PRACTICAL APPROACH TO STUDYING STROKE WITH MICROARRAY - R. W. Gilbert, W. J. Costain, and H. A. Robertson.- YEAST TWO-HYBRID STUDIES - M. J. Smith, K. Pozo, and F. A. Stephenson.- PROTEIN ENGINEERING: CHIMERAGENESIS AND SITE-DIRECTED MUTAGENESIS - M. Davies.- CYSTEINE SCANNING MUTAGENESIS: MAPPING BINDING SITES OF LIGAND-GATED ION CHANNELS - J. G. Newell and C. Czajkowski.- PROTEIN X-RAY CRYSTALLOGRAPHY - D. A. R. Sanders.