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This new volume of Methods in Cell Biology looks at Tetrahymena thermophila, a model organism in experimental biology. Covering sections on Systems perspectives and Operating principles, chapters are written by experts in the field.

With cutting edge material, this comprehensive collection is intended to guide researchers of Tetrahymena thermophila for years to come.

Tetrahymena Thermophila

Part I: Introduction

1. Perspectives on the ciliated protozoan Tetrahymena thermophila

Part II: Systems perspectives

2. The life and times of Tetrahymena
3. Nuclear dualism.
4. Whole Genome Studies of Tetrahymena
5. From Molecules to Morphology: Cellular Organization of Tetrahymena thermophila
6. Conservation and innovation in Tetrahymena membrane traffic: proteins, lipids, and compartments
7. Developmental progression of Tetrahymena through the cell cycle and conjugation

Part III: Operating Principles

8. Tetrahymena in the Laboratory: Strain Resources, Methods for Culture, Maintenance, and Storage
9. Natural populations and inbred strains of Tetrahymena
10. Tetrahymena thermophila Genetics: Concepts and Applications
11. Transformation and strain engineering of Tetrahymena
12. Biochemical approaches including the design and use of strains expressing epitope-tagged proteins
13. Cytological Analysis of Tetrahymena thermophila
14. Purification Of Tetrahymena Cytoskeletal Proteins
15. Behavioral Bioassays and their Uses in Tetrahymena.
16. Tetrahymena in the Classroom