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This volume details the phenotypic characterization of Staphylococcus aureus, with a focus on in vitro and ex vivo methodologies. The chapters in this book cover topics such as in vitro assessment of classical S. aureus virulence attributes; quantifying promoter activity using a S. aureus codon-optimized lacZ plasmid; biologically-relevant growth environments; metabolic and stress resistance assays; and in vivo and ex vivo models of host-pathogen interaction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Staphylococcus aureus: Methods and Protocols is a valuable resource for anyone interested in this fascinating and developing field.

Staphylococcus aureus: Methods and Protocols

Preface...Table of Contents...Contributing Authors...
1. Measurement of Staphylococcus aureus Pigmentation by Methanol ExtractionLeia E. Sullivan and Kelly C. Rice
2. Analysis of Murein Hydrolases and Proteases through ZymographyNichole A. Seawell and Jeffrey L. Bose
3. Detection and Quantification of Secreted Nuclease Activity in Staphylococcus aureus Culture SupernatantsRichard E. Wiemels, Rebecca A. Keogh, and Ronan K. Carroll
4. Quantitative Hemolysis AssaysMiranda J. Ridder, Seth M. Daly, Pamela R. Hall, and Jeffrey L. Bose
5. In Vitro Assay for Quantifying Clumping of Staphylococcus aureus Heidi A. Crosby, Jakub M. Kwiecinski, and Alexander R. Horswill
6. Measuring Staphylococcal Promoter Activities using a Codon-Optimized beta-galactosidase ReporterChristina N. Krute, Nichole A. Seawell, and Jeffrey L. Bose
7. Evaluation of Staphylococcus aureus Antibiotic Tolerance using Kill Curve AssaysJessica N. Brandwein and Kelly C. Rice
8. Fluorescence Polarization (FP) Assay for Measuring Staphylococcus aureus Membrane FluidityKiran B. Tiwari, Suranjana Sen, Craig Gatto, and Brian J. Wilkinson
9. Quantification of Staphylococcus aureus Biofilm Formation by Crystal Violet and Confocal Microscopy Adam B. Grossman, Dylan J. Burgin, and Kelly C. Rice
10. Growth of Staphylococcus aureus using a Rotary Cell Culture System Matthew R. Hauserman and Kelly C. Rice 

11. Time-Resolved Fluorescence Assay for Measuring Oxygen Consumption Rates in Staphylococcus aureusLogan L. Bulock, Jennifer L. Endres, and Marat R. Sadykov
12. Quantifying Staphylococcus aureus Membrane Potential using Flow CytometryNeal D. Hammer
13. Best Practices for Preparation of Staphylococcus aureus Metabolomics Samples Kimberly L. James and Kelly C. Rice
14. A Rat Model of Orthopedic Implant-Associated Infection for Identification of Staphylococcal Biofilm ProteinsMei G. Lei, Ravi Kr. Gupta, and Chia Y. Lee
15. An Ex Vivo Model for Assessing Growth and Survivability of Staphylococcus aureus in Whole Human Blood Brittney D. Gimza, Stephanie M. Marroquin, and Lindsey N. Shaw
16. Bone Marrow-Derived Macrophage Infection AssayMiranda J. Ridder, Mary A. Markiewicz, and Jeffrey L. Bose
17. Measurement of Osteoblast Cytotoxicity Induced by S. aureus Secreted ToxinsCaleb Ford and James E. Cassat
18. Human Skin In Vitro Colonization Model for a Skin Wound Infected by Staphylococcus aureus BiofilmJenelle Chapman and Michael E. Olson
Subject Index List...