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With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology(TM) series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users.

Flow Cytometry Protocols

1. Flow Cytometry: An IntroductionAlice L. Givan2. Breaking the Dimensionality BarrierC. Bruce Bagwell3. Quantitative Fluorescence Measurements with Multicolor Flow CytometryLili Wang, Adolfas K. Gaigalas, and Ming Yan4. Quantum Dots for Quantitative Flow CytometryYang Wu, Larry A. Sklar, and Tione Buranda5. Bead-Based Multiplexed Analysis of Analytes by Flow CytometryHenri C. van der Heyde and Irene Gramaglia6. Flow-Based Combinatorial Antibody Profiling: An Integrated Approach to Cell CharacterizationShane Bruckner, Ling Wang, Ruiling Yuan, Perry Haaland, and Amitabh Gaur7. Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow CytometryJoseph D. Tario, Jr., Katharine A. Muirhead, Dalin Pan, Mark E. Munson, and Paul K. Wallace8. Multiparameter Intracellular Cytokine StainingPatricia Lovelace and Holden T. Maecker9. Phospho Flow Cytometry Methods for Analysis of Kinase Signaling in Cell Lines and Primary Human Blood SamplesPeter O. Krutzik, Angelica Trejo, Kenneth R. Schulz, and Garry P. Nolan10. Multiparametric Analysis of Apoptosis by Flow CytometryWilliam G. Telford, Akira Komoriya, Beverly Z. Packard, and C. Bruce Bagwell11. Multiparameter Cell Cycle AnalysisJames W. Jacobberger, R. Michael Sramkoski, and Tammy Stefan12. Rare Event Detection and Analysis in Flow Cytometry: Bone Marrow Mesenchymal Stem Cells, Breast Cancer Stem/Progenitor Cells in Malignant Effusions, and Pericytes in Disaggregated Adipose TissueLudovic Zimmerlin, Vera S. Donnenberg, and Albert D. Donnenberg13. Flow Cytometric-Based Identification of Immature Myeloerythroid DevelopmentCornelis J.H. Pronk and David Bryder14. Flow Cytometry Immunophenotyping of Hematolymphoid NeoplasiaKatherine R. Calvo, Catharine S. McCoy, and Maryalice Stetler-Stevenson15. Flow Cytometry Assays in Primary Immunodeficiency DiseasesMaurice R.G. O'Gorman, Joshua Zollett, and Nicolas Bensen16. Flow Cytometric Analysis of MicroparticlesHenri C. van der Heyde, Irene Gramaglia, Valéry Combes, Thaddeus C. George, and Georges E. Grau17. Noncytotoxic DsRed Derivatives for Whole-Cell LabelingRita L. Strack, Robert J. Keenan, and Benjamin S. Glick18. Flow Cytometric FRET Analysis of Protein InteractionGyörgy Vereb, Péter Nagy, and János Szöllösi19. Fluorescent Protein-Assisted Purification for Gene Expression ProfilingM. Raza Zaidi, Chi-Ping Day, and Glenn Merlino20. Multiparametric Analysis, Sorting, and Transcriptional Profiling of Plant Protoplasts and Nuclei According to Cell TypeDavid W. Galbraith, Jaroslav Janda, and Georgina M. Lambert21. Lentiviral Fluorescent Protein Expression Vectors for Biotinylation ProteomicsIrene Riz, Teresa S. Hawley, and Robert G. Hawley22. Standard Practice for Cell Sorting in a BSL-3 FacilityStephen P. Perfetto, David R. Ambrozak, Richard Nguyen, Mario Roederer, Richard A. Koup, and Kevin L. Holmes23. The Cytometric Future: It Ain't Necessarily Flow!Howard M. Shapiro