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Chronic pain is a complex phenomenon, which continues to remain undertreated in the majority of affected patients thus representing a significant unmet medical need, but the development of cellular, subcellular, and molecular methods of approaching this epidemic of pain shows great promise. In Analgesia: Methods and Protocols, experts in the field present thorough coverage of molecular analgesia research methods from target discovery through target validation and clinical testing to tolerance and dependence, with extensive chapters on emerging receptor classes as targets for analgesic drugs and innovative analgesic strategies. As a volume in the highly successful Methods in Molecular Biology(TM) series, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections with tips on troubleshooting and avoiding known pitfalls.

Comprehensive and essential, Analgesia: Methods and Protocols promises to aid and enrich the research of all those scientists and clinicians who are interested in what the increasingly molecular future has in store for analgesia research, from the molecular research bench through the animal laboratory to the bedside.

Analgesia: Methods and Protocols

1. Alternatives to Mammalian Pain Models 1: Use of C. elegans for the Study of Volatile AnestheticsLouise M. Steele, Margaret M. Sedensky, and Phil G. Morgan2. Alternatives to Mammalian Pain Models 2: Using Drosophila to Identify Novel Genes Involved in NociceptionJason C. Caldwell and W. Daniel Tracey, Jr.3. Animal Models of Acute Surgical PainHyangin Kim, Backil Sung, and Jianren Mao4. Animal Models of Acute and Chronic Inflammatory and Nociceptive PainJanel M. Boyce-Rustay, Prisca Honore, and Michael F. Jarvis5. Noxious Heat Threshold Measured with Slowly Increasing Temperatures: Novel Rat Thermal Hyperalgesia ModelsKata Bölcskei, Gábor Petho, and János Szolcsányi6. Locomotor Activity in a Novel Environment as a Test of Inflammatory Pain in RatsDavid J. Matson, Daniel C. Broom, and Daniel N. Cortright7. Rationale and Methods for Assessment of Pain-Depressed Behavior in Preclinical Assays of Pain and AnalgesiaS. Stevens Negus, Edward J. Bilsky, Gail Pereira Do Carmo, and Glenn W. Stevenson8. Animal Models of Orofacial PainAsma Khan and Kenneth M. Hargreaves9. Migraine ModelsSilvia Benemei, Francesco De Cesaris, Paola Nicoletti, Serena Materazzi, Romina Nassini, and Pierangelo Geppetti10. Experimental Models of Visceral PainMia Karpitschka and Martin E. Kreis11. Human Correlates of Animal Models of Chronic PainArpad Szallasi12. Human Experimental Pain Models 1: The Ultraviolet Light UV-B Pain ModelJames Modir and Mark Wallace13. Human Experimental Pain Models 2: The Cold Pressor ModelJames Modir and Mark Wallace14. Human Experimental Pain Models 3: Heat/Capsaicin Sensitization and Intradermal Capsaicin ModelsJames Modir and MarkWallace15. The Value of the Dental Impaction Pain Model in Drug DevelopmentStephen A. Cooper and Paul J. Desjardins16. Live Cell Imaging for Studying G Protein-Coupled Receptor Activation in Single CellsDeepak Kumar Saini and Narasimban Gautam17. Recombinant Cell Lines Stably Expressing Functional Ion ChannelsFlorian Steiner, Sraboni Ghose, and Urs Thomet18. Ion Channels in Analgesia ResearchTamara Rosenbaum, Sidney A. Simon, and Leon D. Islas19. Electrophysiological and Neurochemical Techniques to Investigate Sensory Neurons in Analgesia ResearchAlexandru Babes, Michael J.M. Fischer, Gordon Reid, Susanne K. Sauer, Katharina Zimmermann, and Peter W. Reeh20. The Genetics of Pain and Analgesia in Laboratory AnimalsWilliam R. Lariviere and Jeffrey S. Mogil21. RT-PCR Analysis of Pain Genes: Use of Gel-Based RT-PCR for Studying Induced and Tissue-Enriched Gene ExpressionKendall Mitchell and Michael J. Iadarola22. Gene-Based Approaches in the Study of Pathological PainElisa Dominguez, Alice Meunier, and Michel Pohl23. Linkage Analysis and Functional Evaluation of Inherited Clinical Pain ConditionsJohannes J. Krupp, Dennis Hellgren, and Anders B. Eriksson24. Rat Bone Marrow Stromal Cells and Oligonucleotides in Pain ResearchMaría Florencia Coronel, Norma Alejandra Chasseing, and Marcelo José Villar25. Transplantation of Human Mesenchymal Stem Cells in the Study of Neuropathic PainDario Siniscalco26. Delivery of RNA Interference to Peripheral Neurons in vivo Using Herpes Simplex VirusAnna-Maria Anesti27. Combination of Cell Culture Assays and Knockout Mouse Analyses for the Study of Opioid Partial AgonismSoichiro Ide, Masabumi Minami, Ichiro