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One of the striking findings of modern developmental biology has been the high degree of conservation of signaling and developmental mechanisms amongst different animal species. Such conservation allows information learned from a given organism to be applicable to other species, including humans, and has validated the use of a few model systems to deduce general biological principles. In spite of this underlying conservation, however, each species has unique characteristics arising from its evolutionary history. Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods attempts to address the increasingly important need of straddling species boundaries in the context of a single research program by compiling research protocols used in a wide range of vertebrate species. In fact, this volume has been designed so that readers can readily find information on species other than the one with which they may be most familiar. These protocols include not only embryological methods, but also cellular and genetic approaches that have complemented and expanded our understanding of embryonic development. In addition, a number of chapters highlight a specific method that is in principle applicable to multiple species, such as TILLING and ZFN-mediated mutagenesis, the generation of Embryonic Stem (ES) cell lines, and nuclear/oocyte transfer. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods serves as an ideal guide to the molecular, cell, and developmental biology community and will hopefully contribute to the ongoing collective effort towards a better understanding of the beauty and logic of vertebrate development.

Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods

1. Approaches and Species in the History of Vertebrate Embryology

Nick Hopwood

2. Manipulating and Imaging the Early Xenopus laevis Embryo

Michael V. Danilchik

3. Manipulation of Gene Function in Xenopus laevis

Mizuho S. Mimoto and Jan L. Christian

4. Developmental Genetics in Xenopus tropicalis

Timoty J. Geach and Lyle B. Zimmerman

5. Embryological and Genetic Manipulation of Chick Development

Laura S. Gammill and Catherine E. Krull

6. Embryological Manipulations in Zebrafish

Yuhua Sun, Dorota Wloga, and Scott T. Dougan

7. Practical Approaches for Implementing Forward Genetic Strategies in Zebrafish

Sreelaja Nair and Francisco Pelegri

8. Essential Techniques for Introducing Medaka to a Zebrafish Laboratory - Toward the Combined Use of Medaka and Zebrafish for Further Genetic Dissection of the Function of the Vertebrate Genome

Sean R. Porazinski, Huijia Wang, and Makoto Furutani-Seiki

9. Ex-utero Culture and Imaging of Mouse Embryos

Anna Piliszek, Gloria S. Kwon, and Anna-Katerina Hadjantonakis

10. Detection of Gene Expression in Mouse Embryos and Tissue Sections

Edwina McGlinn and Jennifer H. Mansfield

11. Gene Targeting in the Mouse

Anne E. Griep, Manorama C. John, Sakae Ikeda, and Akihiro Ikeda

12. Creating a "Hopeful Monster": Mouse Forward Genetic Screens

Vanessa L. Horner and Tamara Caspary

13. Assisted Reproductive Technology in Nonhuman Primates

Tien-cheng Chang and Anthony W.S. Chan

14. Embryological Methods in Ascidians: the Villefranche-sur-Mer Protocols

Christian Sardet, Alex McDougall, Hitoyoshi Yasuo, Janet Chenevert, Gérard Pruliere, Rémi Dumollard, Clare Hudson, Celine Hebras, Ngan Le Nguyen and Alexandre Paix

15. Ciona Genetics

Michael T. Veeman, Shota Chiba and William C. Smith

16. Analyses of Gene Function in Amphioxus Embryos by Microinjection of mRNAs and Morpholino-oligonucleotides

Linda Z. Holland and Takayuki Onai

17. Reptile Embryology

Matthew K. Vickaryous and Katherine E. McLean

18. Reproductive and Developmental Manipulation of the Marsupial, the Tammar Wallaby Macropus eugenii

Marylin B. Renfree and Andrew J. Pask

19. Mutant Generation in Vertebrate Model Organisms by TILLING

Sylke Winkler, Nicola Gscheidel, and Michael Brand

20. Inducing High Rates of Targeted Mutagenesis in Zebrafish using Zinc Finger Nucleases (ZFNs)

Jasmine M. McCammon, Yannick Doyon, and Sharon L. Amacher

21. Derivation of Mouse Embryonic Stem Cell Lines from Blastocysts Produced by Fertilization and Somatic Cell Nuclear Transfer

Zhongde Wang

22. Cloning Mice and ES Cells by Nuclear Transfer from Somatic Cells and Fully Differentiated Cells

Zhongde Wang

23. Keeping Two Animal Systems in One Lab: A Frog Plus Fish Case Study

Hazel Sive

24. Laboratory Guidelines for Animal Care

Marcelo Cuoto