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This volume provides comprehensive knowledge on the fundamental techniques and detailed laboratory protocols used in stem cell research and their potential application in the field of neuroscience. The chapters in this book cover detailed descriptions of techniques for isolation, maintenance, differentiation, and characterization of neural stem cells, and the development of in vitro stem cell-based neurological disease model. There are detailed methods on leading techniques for stem cell genetic engineering and intra-organ transplantation for research and therapeutic purposes, in vivo imaging of donor stem cells, and essential requirements for setting up a new stem cell laboratory. Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals.

Cutting-edge and pragmatic, Stem Cell Technologies in Neuroscience is a valuable resource for academic and translational researchers, industry scientists, biology teachers, and life science graduate students to grasp the vital pulse of the rapidly growing field of stem cell research.

 

Stem Cell Technologies in Neuroscience

Neuromethods Series Preface...
Preface...
Table of Contents...
Contributing Authors...

1.  Three-Dimensional Cultures of Human Neural Stem Cells: An Application for Modeling Alzheimer's Disease Pathogenesis
Se Hoon Choi, Carla D'Avanzo, Young Hye Kim, Enjana Bylykbashi, Matthias Hebisch, Oliver Brüstle, Ruldolph E. Tanzi, and Doo Yeon Kim

2. Neural Stem Cell Fate Control on Micropatterned Substrates
Leonora Buzanska, Marzena Zychowicz, Ana Ruiz, and François Rossi

3. Dopaminergic and GABAergic Neuron In Vitro Differentiation from Embryonic Stem Cells
Talita Glaser, Juliana Correa-Velloso, Ágatha Oliveira, Yang D. Teng, and Henning Ulrich

4. Transfection of Cultured Primary Neurons
Annalisa Rossi, Ralf Dahm, and Paolo Macchi

5.  Reprogramming of Mouse Fibroblasts to Induced Oligodendrocyte Progenitor Cells
Robert T. Karl, Angela M. Lager, Fadi J. Najm, and Paul J. Tesar

6. Isolation and Culture of Oligodendrocyte Precursor Cells from Pre- and Postnatal Rodent Brain
Danyang He, Bradley Meyer, and Q. Richard Lu

7. Schwann Cell Isolation and Culture Reveals the Plasticity of these Glia
David E. Weinstein

8. Generation of Cerebral Organoids Derived from Human Pluripotent Stem Cells
Mark E. Hester and Alexis B. Hood

9. Development of Mouse Cell Based In Vitro Blood-Brain Barrier Models
Malgorzata Burek, Ellaine Salvador, and Carola Y. Förster

10. Microfluidic Device for Studying Traumatic Brain Injury
Yiing C. Yap, Tracey C. Dickson, Anna E. King, Michael C. Breadmore, and Rosanne M. Guijt

11. Multimodal Neural Stem Cell Research Protocols for Experimental Spinal Cord Injuries
Yang D. Teng, Evan Y. Snyder, Xiang Zeng, Jamie E. Anderson, and Inbo Han
12.  Real-Time Dual MRI for Predicting and Subsequent Validation of Intra-Arterial Stem Cell Delivery to the Central Nervous System
Piotr Walczak and Miroslaw Janowski

13. Standardized Cryopreservation of Stem Cells
Maria L. Thompson, Eric J. Kunkel, and Rolf O. Ehrhardt

14. Isolation and Characterization of Extracellular Vesicles in Stem Cell-Related Studies
Zezhou Zhao, Dillon C. Muth, Vasiliki Machairaki, Linzhao Cheng, Kenneth W. Witwer

15. Essential Requirements for Setting Up a Stem Cell Laboratory
Philip H. Schwartz and Robin L. Wesselschmidt