Książki

Chronic pain is a complex phenomenon, which continues to remain undertreated in the majority of affected patients thus representing a significant unmet medical need, but the development of cellular, subcellular, and molecular methods of approaching this epidemic of pain shows great promise. In Analgesia: Methods and Protocols, experts in the field present thorough coverage of molecular analgesia research methods from target discovery through target validation and clinical testing to tolerance and dependence, with extensive chapters on emerging receptor classes as targets for analgesic drugs and innovative analgesic strategies. As a volume in the highly successful Methods in Molecular Biology(TM) series, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections with tips on troubleshooting and avoiding known pitfalls.

Comprehensive and essential, Analgesia: Methods and Protocols promises to aid and enrich the research of all those scientists and clinicians who are interested in what the increasingly molecular future has in store for analgesia research, from the molecular research bench through the animal laboratory to the bedside.

Analgesia: Methods and Protocols

1. Alternatives to Mammalian Pain Models 1: Use of C. elegans for the Study of Volatile Anesthetics
Louise M. Steele, Margaret M. Sedensky, and Phil G. Morgan

2. Alternatives to Mammalian Pain Models 2: Using Drosophila to Identify Novel Genes Involved in Nociception
Jason C. Caldwell and W. Daniel Tracey, Jr.

3. Animal Models of Acute Surgical Pain
Hyangin Kim, Backil Sung, and Jianren Mao

4. Animal Models of Acute and Chronic Inflammatory and Nociceptive Pain
Janel M. Boyce-Rustay, Prisca Honore, and Michael F. Jarvis

5. Noxious Heat Threshold Measured with Slowly Increasing Temperatures: Novel Rat Thermal Hyperalgesia Models
Kata Bölcskei, Gábor Petho, and János Szolcsányi

6. Locomotor Activity in a Novel Environment as a Test of Inflammatory Pain in Rats
David J. Matson, Daniel C. Broom, and Daniel N. Cortright

7. Rationale and Methods for Assessment of Pain-Depressed Behavior in Preclinical Assays of Pain and Analgesia
S. Stevens Negus, Edward J. Bilsky, Gail Pereira Do Carmo, and Glenn W. Stevenson

8. Animal Models of Orofacial Pain
Asma Khan and Kenneth M. Hargreaves

9. Migraine Models
Silvia Benemei, Francesco De Cesaris, Paola Nicoletti, Serena Materazzi, Romina Nassini, and Pierangelo Geppetti

10. Experimental Models of Visceral Pain
Mia Karpitschka and Martin E. Kreis

11. Human Correlates of Animal Models of Chronic Pain
Arpad Szallasi

12. Human Experimental Pain Models 1: The Ultraviolet Light UV-B Pain Model
James Modir and Mark Wallace

13. Human Experimental Pain Models 2: The Cold Pressor Model
James Modir and Mark Wallace

14. Human Experimental Pain Models 3: Heat/Capsaicin Sensitization and Intradermal Capsaicin Models
James Modir and MarkWallace

15. The Value of the Dental Impaction Pain Model in Drug Development
Stephen A. Cooper and Paul J. Desjardins

16. Live Cell Imaging for Studying G Protein-Coupled Receptor Activation in Single Cells
Deepak Kumar Saini and Narasimban Gautam

17. Recombinant Cell Lines Stably Expressing Functional Ion Channels
Florian Steiner, Sraboni Ghose, and Urs Thomet

18. Ion Channels in Analgesia Research
Tamara Rosenbaum, Sidney A. Simon, and Leon D. Islas

19. Electrophysiological and Neurochemical Techniques to Investigate Sensory Neurons in Analgesia Research
Alexandru Babes, Michael J.M. Fischer, Gordon Reid, Susanne K. Sauer, Katharina Zimmermann, and Peter W. Reeh

20. The Genetics of Pain and Analgesia in Laboratory Animals
William R. Lariviere and Jeffrey S. Mogil

21. RT-PCR Analysis of Pain Genes: Use of Gel-Based RT-PCR for Studying Induced and Tissue-Enriched Gene Expression
Kendall Mitchell and Michael J. Iadarola

22. Gene-Based Approaches in the Study of Pathological Pain
Elisa Dominguez, Alice Meunier, and Michel Pohl

23. Linkage Analysis and Functional Evaluation of Inherited Clinical Pain Conditions
Johannes J. Krupp, Dennis Hellgren, and Anders B. Eriksson

24. Rat Bone Marrow Stromal Cells and Oligonucleotides in Pain Research
María Florencia Coronel, Norma Alejandra Chasseing, and Marcelo José Villar

25. Transplantation of Human Mesenchymal Stem Cells in the Study of Neuropathic Pain
Dario Siniscalco

26. Delivery of RNA Interference to Peripheral Neurons in vivo Using Herpes Simplex Virus
Anna-Maria Anesti

27. Combination of Cell Culture Assays and Knockout Mouse Analyses for the Study of Opioid Partial Agonism
Soichiro Ide, Masabumi Minami, Ichiro