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Leading experts in nanobiotechnology comprehensively review the most recent advances in instrumentation and methodology, as well as their applications in genomics and proteomics. The authors provide a wide variety of techniques and methods for dealing with protein functions and structures at the nanoscale level, including nanostructured systems, nanomaterials, carbon nanotubes and nanowires, optical nanosensors, and nanoelectrodes. Among the highlights are techniques for the in vivo tracking of biochemical processes using fluorescent molecular probes and nanosensors, and the exploration of biochemical processes and submicroscopic structures of living cells at unprecedented resolutions using near-field optics. Also discussed is the development of nanocarrier methodology for the targeted delivery of drugs whose shells are conjugated with antibodies for targeting specific antigens. Rapidly emerging at the intersection of nanotechnology, materials science, and molecular biology, the field of nanobiotechnology promises to elucidate many life processes at the molecular-level previously invisible to human inquiry, and thereby dramatically to transform diagnostics, therapy, and drug discovery in this postgenomic world. In Protein Nanotechnology: Protocols, Instrumentation, and Applications, leading experts in nanobiotechnology comprehensively review the most recent advances in instrumentation and methodology, as well as their applications in genomics and proteomics. The authors provide a wide variety of techniques and methods for dealing with protein functions and structures at the nanoscale level, including nanostructured systems, nanomaterials, carbon nanotubes and nanowires, optical nanosensors, and nanoelectrodes. Among the highlights are techniques for the in vivo tracking of biochemical processes using fluorescent molecular probes and nanosensors, and the exploration of biochemical processes and submicroscopic structures of living cells at unprecedented resolutions using near-field optics. Also discussed is the development of nanocarrier methodology for the targeted delivery of drugs whose shells are conjugated with antibodies for targeting specific antigens. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Protein Nanotechnology: Protocols, Instrumentation, and Applications provides investigators a wide variety of readily reproducible techniques and methods for exploring both protein functions and structures at the nanoscale level and their powerful new biological and medical applications.

Protein Nanotechnology: Protocols, Instrumentation, and Applications

Protein Nanotechnology: The New Frontier in Biosciences
Tuan Vo-Dinh

Kinetics and Mechanisms of Protein Crystallization at the Molecular Level
Peter G. Vekilov

Nanostructured Systems for Biological Materials
Esther H. Lan, Bruce Dunn, and Jeffrey I. Zink

Nanomaterials of Drug Delivery Systems for Tissue Regeneration
Yasuhiko Tabata

Nanotechnology With S-Layer Proteins
Bernhard Schuster, Erika Györvary, Dietmar Pum, and Uwe B. Sleytr

Folding of b-Structured Fibrous Proteins and Self-Assembling Peptides
Anna Mitraki and Mark J. van Raaij

Application of NMR Methods to Identify Detection Reagents for Use in Development of Robust Nanosensors
Monique Cosman, Viswanathan V. Krishnan, and Rod Balhorn

Studying 3D Subdomains of Proteins at the Nanometer Scale Using Fluorescence Spectroscopy
Pierre M. Viallet and Tuan Vo-Dinh

Carbon Nanotubes and Nanowires for Biological Sensing
Jun Li, Hou Tee Ng, and Hua Chen

Carbon Nanotube Systems to Communicate With Enzymes
J. Justin Gooding and Joe G. Shapter

Molecularly Imprinted Polymers for Biomolecular Recognition
Alexandra Molinelli, Markus Janotta, and Boris Mizaikoff

Plasmonics-Based Nanostructures for Surface-Enhanced Raman Scattering Bioanalysis
Tuan Vo-Dinh, Fei Yan, and David L. Stokes

Bacterial Virus f29 DNA-Packaging Motor and Its Potential Applications in Gene Therapy and Nanotechnology
Peixuan Guo

Construction of Ordered Protein Arrays
Jarrod Clark, Taras Shevchuk, Piotr M. Swiderski, Rajesh Dabur, Laura E. Crocitto, Yaroslav I. Buryanov, and Steven S. Smith

Bioengineering and Characterization of DNA-Protein Assemblies Floating on Supported Membranes
Wilfrid Boireau, Anthony C. Duncan, and Denis Pompon

Nanosystems for Biosensing: Multianalyte Immunoassay on a Protein Chip
EiichiTamiya, Zheng-liang Zhi, Yasutaka Morita, and Quamrul Hasan

Optical Nanosensors for Detecting Proteins and Biomarkers in Individual Living Cells
Tuan Vo-Dinh

Nanoelectrodes Integrated in Atomic Force Microscopy Cantilevers for Imaging of In Situ Enzyme Activity
Angelika Kueng, Christine Kranz, Alois Lugstein, Emmerich Bertagnolli, and Boris Mizaikoff

Protein Amyloidose Misfolding: Mechanisms, Detection, and Pathological Implications
Nigil Satish Jeyashekar, Ajit Sadana, and Tuan Vo-Dinh

Near-Field Scanning Optical Microscopy for Bioanalysis at Nanometer Resolution
Musundi B. Wabuyele, Mustafa Culha, Guy D. Griffin, Pierre M. Viallet, and Tuan Vo-Dinh

Index