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As experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly became a necessity. Angiogenesis Protocols, Second Edition remains true to its original vision of providing a single source for angiogenesis researchers, irrespective of levels of resource and expertise, by collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro. This information, however, is expanded to include chapters on circulating endothelial progenitor cells, angiogenic signalling pathways, imaging of angiogenesis, and measurement of tissue blood flow. Written in the Methods in Molecular Biology(TM) series format, the chapters provide step-by-step laboratory protocols, lists of necessary materials and reagents, and a Notes section, which details tips on troubleshooting and avoiding known pitfalls.

Extensive and cutting-edge, Angiogenesis Protocols, Second Edition is not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis.

Angiogenesis Protocols

Section I: Review ArticlesChapter 1. Angiogenic Signalling PathwaysZubair Ahmed and Roy BicknellChapter 2. Imaging AngiogenesisNatalie Charnley, Stephanie Donaldson, and Pat PriceSection II: Microscopic AssessmentChapter 3. Assessing Tumor Angiogenesis in Histological SamplesStephen B FoxChapter 4. Immunohistochemical Methods for Measuring Tissue LymphangiogenesisSteven Clasper and David G. JacksonSection III: Cell Isolation TechniquesChapter 5. Vascular Endothelial Cells from Human Micro- and Macro-vessels: Isolation, Characterisation and CulturePeter W HewettChapter 6. Lymphatic Endothelial Cells: Establishment of Primaries and Characterization of Established LinesRiccardo E. Nisato and Michael S. PepperChapter 7. Isolation, Culture and Characterisation of Vascular Smooth Muscle CellsAdrian T Churchman and Richard CM SiowChapter 8. Circulating Endothelial Progenitor Cells (CEPC)Michael C. Schmid and Judith A. VarnerSection IV: In vitro TechniquesChapter 9. In vitro Assays for Endothelial Cell Functions Related to Angiogenesis: Proliferation, Motility, Tubular Differentiation and ProteolysisSuzanne A Eccles, William Court, Lisa Patterson and Sharon SandersonChapter 10. Tube Formation: an In Vitro Matrigel Angiogenesis AssayM. Lourdes PonceChapter 11. Three-dimensional In vitro Angiogenesis in the Rat Aortic Ring ModelDavid C. West and Mike F. BurbridgeChapter 12. Static and Dynamic Assays of Cell Adhesion Relevant to the VasculatureLynn M. Butler, Helen M. McGettrick, and Gerard B. NashChapter 13. Genetic Manipulation of Corneal Endothelial Cells: Transfection and Viral TransductionEckart BertelmannChapter 14. Creation of Human Skin Equivalents for the In vitro Study of Angiogenesis in Wound HealingIra M. Herman and Alice LeungSection V: In vivo TechniquesChapter 15. Measurement of Angiogenic Phenotype by Use of a Two Dimensional Mesenteric Angiogenesis AssayAndrew V Benest and David O BatesChapter 16. Quantitative Estimation of Tissue Blood Flow RateGillian M Tozer, Vivien E Prise and Vincent J CunninghamChapter 17. In Vivo Matrigel Migration and Angiogenesis AssayKatherine M. MalindaChapter 18. The Sponge Implant Model of AngiogenesisSilvia Passos Andrade and Mônica Alves Neves Diniz FerreiraChapter 19. The Dorsal Skinfold Chamber: Studying Angiogenesis by Intravital MicroscopyAxel Sckell and Michael LeunigChapter 20. The Corneal Pocket AssayMarina Ziche and Lucia MorbidelliChapter 21. Use of the Hollow Fibre Assay for Studies on Tumor NeovasculatureSteven D. ShnyderChapter 22. The Cranial Bone Window Model: Studying Angiogenesis of Primary and Secondary Bone Tumors by Intravital MicroscopyAxel Sckell and Frank M. Klenke