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Whole genome amplification generates microgram quantities of genomic DNA starting from as little as a few femtograms and is a vital technique when sample material is limited. Whole Genome Amplification: Methods Express is a comprehensive up-to-date laboratory manual for this key technique.

Whole Genome Amplification

1. Introduction to whole genome amplification (Jose M. Lage and Paul M. Lizardi)



2. Single nucleotide polymorphism typing using degenerate- oligonucleotide-primed PCR-amplified products (Makoto Bannai and Katsushi Tokunaga)



3. Whole genome amplification by improved primer-extension pre-amplification PCR (Peter J. Wild and Wolfgang Dietmaier)



4. Global amplification using SCOMP: single-cell comparative genomic hybridization (Nona C.R. Arneson, Arezou A. Ghazani, et al.)



5. PRSG, a whole genome amplification method based on adaptor-ligation PCR of randomly sheared genomic DNA (Hiroki Sasaki and Kazuhiko Aoyagi)



6. GenomePlex whole genome amplification (Simon Hughes, Gabrielle Sellick, et al.)



7. DNA linear amplification (Chih Long Liu, Bradley E. Bernstein and Stuart L. Schreiber)



8. Multiple displacement amplification of genomic DNA (Roger S. Lasken)



9. Multiple displacement amplification from single bacterial cells (Roger S. Lasken, et al.)



10. Genome amplification tolerant to sample degradation: application to formalin-fixed, paraffin-embedded specimens (G. Mike Makrigiorgos)



11. Pre-implantation genetic diagnosis using whole genome amplification (Alan H. Handyside, Mark D. Robinson and Francesco Fiorentino)



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Index