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This handy guide provides detailed practical information for HPLC practitioners of all skill levels, enabling them to optimize the performace of their HPLC/UHPLC system for a wide range of analytical tasks.

Optimization in HPLC: Concepts and Strategies

PART 1: OPTIMIZATION STRATEGIES FOR DIFFERENT MODES AND USES OF HPLCWorking under regulatory constraintsReverse phase gradient methods2-Dimensional chromatographyHILICLC-MSBiomoleculesSupercritical fluid chromatographyChiral substancesColumn selection for small moleculesOptimization based on analyte structurePART 2: COMPUTER-AIDED OPTIMIZATIONChromSwordDryLabFusion QbDQuality by Design approaches to determine the robust rangePART 3: CURRENT CHALLENGES FOR HPLC USERS IN INDUSTRYBig ChemistryBig Analytical Service ProvidersSmall Analytical Service ProvidersPART 4: CURRENT CHALLENGES FOR HPLC EQUIPMENT SUPPLIERSThermo Fisher ScientificAgilentShimadzuWatersKnauer