In the last three decades, the fast development of single-molecule techniques has revolutionized the way we observe and understand biological processes. Some of these techniques have been further adapted as tools for bioanalysis. This book summarizes and details the frontiers of the development of these tools as well as their applications. All chapter contributors are young but established researchers in their respective fields. The main content also forms the lecture notes of a chemistry graduate course in charge of the book editor, Prof. Shuo Huang, at Nanjing University. This book is suitable to be used as a textbook for a high-level undergraduate or an entry-level graduate course. The systematically written content provides a thorough illustration of the mechanisms of each methodology presented.
Single-Molecule Tools for Bioanalysis
1. Single-Molecule Analysis by Biological Nanopores
2. Optical Tweezers for the Manipulation of Single Molecules
3. Single-Molecule Biosensing by Fluorescence Resonance Energy Transfer (FRET)
4. DNA Origamis as Single-Molecule Biosensors
5. Single-Molecule Manipulation by Magnetic Tweezers
6. Long-Time Recording of Single-Molecule Dynamics in Solution by Anti-Brownian Trapping