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This book serves as a guide for identifying and applying commonly used cell-based translational assays as well as for assessing the therapeutic potential of new immuno-oncology therapeutics and advancing their mechanism of action. The detailed chapters within will provide readers with a baseline understanding of the pros and cons as well as key considerations for applying assays that are more reflective of the human immune-tumor microenvironment in order to increase their translatability into the clinic. Written for the Methods in Pharmacology and Toxicology series, the contents of this volume include the kind of specifics and real-world implementation advice to ensure success in the lab.
Authoritative and practical, Immuno-Oncology: Cellular and Translational Approaches aims to aid researchers working on biotechnology and pharmaceutical efforts to search for the next generation of safer and more effective cancer immunotherapeutics.

Immuno-Oncology: Cellular and Translational Approaches

1. An In Vitro System Combining Tumor Cells and Lymphocytes to Predict the In Vivo Response of Immunomodulatory Antibodies

            Min Dai, Ingegerd Hellstrom, and Karl Erik Hellstrom

 

2. High-Throughput Direct Cell Counting Method for Immuno-Oncology Functional Assays Using Image Cytometry

            Leo Li-Ying Chan

 

3. In Vitro Immunotherapy Potency Assays Using Real-Time Cell Analysis

            Biao Xi, Peifang Ye, Vita Golubovskaya, and Yama Abassi

 

4. Assessing the Potency of T Cell-Redirecting Therapeutics Using In Vitro Cancer Cell Killing Assays

            Tomasz Dobrzycki, Andreea Ciuntu, Andrea Stacey, Joseph D. Dukes, and Andrew D. Whale

 

5. Induction and Potential Reversal of a T Cell Exhaustion-Like State: In Vitro Potency Assay for Functional Screening of Immune Checkpoint Drug Candidates

            Eden Kleiman, Wushouer Ouerkaxi, Marc Delcommenne, Geoffrey W. Stone, Paolo Serafini, Mayra Cruz Tleugabulova, and Pirouz M. Daftarian

 

6. Validation of an Image-Based 3D Natural Killer Cell-Mediated Cytotoxicity Assay

            Brad Larson, Lubna Hussain, and Jenny Schroeder

 

7. 3D-3-Culture: Tumor Models to Study Heterotypic Interactions in the Tumor Microenvironment

            Sofia P. Rebelo, Catarina Pinto, Nuno Lopes, Tatiana Martins, Paula Marques Alves, and Catarina Brito

 

8. Considerations in Developing Reporter Gene Bioassays for Biologics

            Jamison Grailer, Richard A. Moravec, Zhijie Jey Cheng, Manuela Grassi, Vanessa Ott, Frank Fan, and Mei Cong

 

9. Miniaturized Single Cell Imaging for Developing Immuno-Oncology Combinational Therapies

            Thomas Jacob, Pavani Malla, and Tania Vu

 

10. Precision Medicine: The Function of Receptor Occupancy in Drug Development

            Leanne Flye-Blakemore, Christele Gonneau, Nithianandan Selliah, Sriram Ramanan, Alan Lackey, and Yoav Peretz

 

11. In Vitro Assays for Assessing Potential Adverse Effects of Cancer Immunotherapeutics

            Jinze Li, Mayur S. Mitra, and Gautham K. Rao

 

12. The Quest for the Next-Generation of Tumor Targets: Discovery and Prioritization in the Genomics Era

            Maurizio Chiriva-Internati, Leonardo Mirandola, Franco Marincola, Gianluca Rotino, Jose A. Figueroa, Fabio Grizzi, and Robert Bresalier