Bookstore

Thisvolume covers multidisciplinary approaches on various aspects of ChikungunyaVirus (CHIKV) research that was brought together from leading laboratoriesacross the globe. Topics covered include techniques in clinical and diagnosticvirology, basic protocols in cell and virus culture, bioinformatics, andproteomics approaches in cellular response studies. Chikungunya Virus:Methods and Protocols also discusses methods in immunology and animal modelstudies, as well as different strategies of antivirals and vaccine developmentfor therapeutics against CHIKV infection. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary material and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.

Thorough and practical, Chikungunya Virus: Methods and Protocols is auseful and comprehensive resource for CHIKV researchers and anyone interestedin learning more about the Chikungunya Virus.

Chikungunya Virus: Methods and Protocols

Part I Clinical and Diagnostic Virology

1. Evolution and Epidemiologyof Chikungunya Virus
Giada Rossini, Maria Paola Landini, andVittorio Sambri

2. Detectionand Quantification of Chikungunya Virus by Real-Time RT-PCR Assay
Seok Mui Wang, Ummul Haninah Ali, ShamalaDevi Sekaran, and Ravindran Thayan

3. Advanced Genetic Methodologiesin Tracking Evolution and Spread of Chikungunya Virus
Hapuarachchige Chanditha Hapuarachchi andKim-Sung Lee

4. SyntheticPeptide-Based Antibody Detection for Diagnosis of Chikungunya Infection withand without Neurological Complications
Rajpal S. Kashyap, Shradha S. Bhullar,Nitin H. Chandak, and Girdhar M. Taori

5. Expression and Purification of E2 Glycoprotein from InsectCells (Sf9) for Use in Serology
Chong Long Chua, I-Ching Sam, and YokeFun Chan

6. Diagnostic Methods for CHIKV Based on Serological Tools
Paolo Gaibani, Maria Poala Landini, andVittorio Sambri

7. Utilization and Assessment of Throat Swab and Urine Specimensfor Diagnosis of Chikungunya Virus Infection
Chandrashekhar G. Raut, H. Hanumaiah, andWrunda C. Raut

Part II Cell Culture, Virus Replication,and Cellular Responses

8. Propagationof Chikungunya Virus Using Mosquito Cells
Swee Kim Ang, Shirley Lam, and JustinJang Hann Chu

9. InfectiousViral Quantification of Chikungunya Virus - Virus Plaque Assay
Parveen Kaur, Regina Ching Hua Lee, andJustin Jang Hann Chu

10. MolecularEpidemiology of Chikungunya Virus by Sequencing
Ravindran Thayan, Mohd Apandi Yusof,Zainah Saat, Shamala Devi Sekaran, and Seok Mui Wang

11. Chikungunya Virus Infection of Aedes Mosquitoes
Hui Vern Wong, Yoke Fun Chan, I-ChingSam, Wan Yusof Wan Sulaiman, and Indra Vythilingam

12. Analysisof CHIKV in Mosquitoes Infected via Artificial Blood Meal
Jeremy P. Ledermann and Ann M. Powers

13. ChikungunyaVirus Growth and Fluorescent Labeling: Detection of Chikungunya Virus by ImmunofluorescenceAssay
Meng Ling Moi and Tomohiko Takasaki

14. Virus Isolation and Preparation of Sucrose-BandedChikungunya Virus Samples for Transmission Electron Microscopy
Chang-Kweng Lim

15. Viral-HostProtein Interaction Studies Using Yeast Two-Hybrid Screening Method
Namrata Dudha and Sanjay Gupta

16. Application of GelC-MS/MS to Proteomic Profiling of Chikungunya Virus Infection:Preparation of Peptides for Analysis
Atchara Paemanee, Nitwara Wikan, SittirukRoytrakul, and Duncan R. Smith

17. BioinformaticsBased Approaches to Study Virus-Host Interactions During Chikungunya Virus Infection
Sreejith Rajasekharan and Sanjay Gupta

18. T-Cell Epitope Prediction ofChikungunya Virus
Christine Loan Ping Eng, Tin Wee Tan, andJoo Chuan Tong

Part III Immunology and Animal ModelStudies

19. MouseModels of Chikungunya Virus
Lara J. Herrero, Penny A. Rudd, XiangLiu, Stefan Wolf, and Suresh Mahalingam

20. Generationof Mouse Monoclonal Antibodies Specific to Chikungunya Virus Using ClonaCell-HYHybridoma Cloning Kit
Chow Wenn Yew and Yee Joo Tan

21. ImmunohistochemicalDetection of Chikungunya Virus Antigens in Formalin-Fixed and Paraffin-EmbeddedTissues
Chun Wei Chiam, I-Ching Sam, Yoke FunChan, Kum Thong Wong, and Kien Chai Ong

Part IV Antivirals and Vaccines

22. Antiviral Strategies against Chikungunya Virus
Rana Abdelnabi, Johan Neyts, and LeenDelang

23. A Real Time Cell Analyzing Assay for Identificationof Novel Antiviral Compounds against Chikungunya Virus
Keivan Zandi

24. Using Bi-Cistronic Baculovirus Expression Vector System to Screen the Compoundsthat Interfere the Infection of Chikungunya Virus
Szu-Cheng Kuo, Chao-Yi Teng, Yi-Jung Ho,Ying-Ju Chen, and Tzong-Yuan Wu

25. NeutralizationAssay for Chikungunya Virus Infection: Plaque Reduction Neutralization Test
Nor Azila Muhammad Azami, Meng Ling Moi,and Tomohiko Takasaki

26. ReverseGenetics Approaches for Chikungunya Virus
Patchara Phuektes and Justin Jang HannChu

27. Productionof Chikungunya Virus-Like Particles and Subunit Vaccines in Insect Cells
Stefan W. Metz and Gorben P. Pijlman

28. Protocols for Developing Novel Chikungunya Virus DNAVaccines
Christopher Chung, Kenneth E. Ugen,Niranjan Y. Sardesai, David B. Weiner, and Kar Muthumani