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Genetic toxicology is recognized by geneticists and researchers concerned with the genetic impact of man-made chemicals. In Genotoxicity Assessment: Methods and Protocols, expert researchers in the field provide comprehensive genetic toxicology protocols. These include in vitro and in vivo protocols on mutation assays, cytogenetic techniques, and primary DNA damage, assays in alternate to animal models, and updated ICH guidelines. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Genotoxicity Assessment: Methods and Protocols seeks to aid research students and scientists working in regulatory toxicology as well as biomedical, biochemical and pharmaceutical sciences.

Genotoxicity Assessment: Methods and Protocols

Part I: Gene Mutation Assays

1. Bacterial Mutation Assays

Errol Zeiger

2. In Vitro Mouse Lymphoma (L5178Y Tk^+/- -3.7.2.C) Forward Mutation Assay

Melissa R. Schisler, Martha M. Moore, and B. Bhaskar Gollapudi

3. Erythrocyte--Based Pig--a Gene Mutation Assay

Jeffrey C. Bemis, Nikki E. Hall, and Stephen D. Dertinger

4. Detection of in vivo Mutation in the Hprt and Pig--a Genes of Rat Lymphocytes

Vasily N. Dobrovolsky, Joseph G. Shaddock, Roberta A. Mittelstaedt, Daishiro Miura, and Robert H. Heflich

5. In vivo cII, gpt, and Spi^- gene Mutation Assays in Transgenic Mice and Rats

Mugimane G. Manjanatha, Xuefei Cao, Sharon D. Shelton, Roberta A. Mittelstaedt, and Robert H. Heflich

Part II; Assays for Chromosomal Abnormalities

6. In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests

Pasquale Mosesso, Serena Cinelli, Adyapalam T. Natarajan, and Fabrizio Palitti

7. Analysis of Chromosome Aberrations in Somatic and Germ Cells of the Mouse

Francesca Pacchierotti and Valentina Stocchi

8. Chromosomal Aberration Test in Human Lymphocytes

Christian Johannes and Guenter Obe

9. In vivo Micronucleus Assay in Mouse Bone Marrow and Peripheral Blood

Sawako Kasamoto, Shoji Masumori, and Makoto Hayashi

10. Micronucleus Assay in Human Cells: Lymphocytes and Buccal Cells

Claudia Bolognesi and Michael Fenech

11. Flow Cytometric Determination of Micronucleus Frequency

Azeddine Elhajouji and Magdalena Lukamowicz-Rajska

12. Fluorescent in situ Hybridization (FISH) Technique for the Micronucleus Test

Ilse Decordier and Micheline Kirsch-Volders

13. Comparative Genomic Hybridization (CGH) Genotoxicology

Baumgartner A

14. The in vitro Micronucleus Assay and Kinetochore Staining: Methodology and Criteria for the Accurate Assessment of Genotoxicity and Cytotoxicity

Bella B. Manshian, Neenu Singh, and Shareen H. Doak

Part III: Tests for Primary DNA Damage

15. g--H2AX Detection in Somatic and Germ Cells of Mice

Eugenia Cordelli and Lorena Paris

16. Multicolour Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers

Julian Laubenthal, Michal R. Gdula , Alok Dhawan, and Diana Anderson

17. The Comet Assay: Assessment of in vitro and in vivo DNA Damage

Mahima Bajpayee, Ashutosh Kumar, and Alok Dhawan

18. The Comet Assay in Human Biomonitoring

Diana Anderson, Alok Dhawan, and Julian Laubenthal

19. The Comet assay in Marine Animals

G. Frenzilli and B.P. Lyons

20. Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo

Fabrice Nesslany

21. ³²P--Postlabeling Analysis of DNA Adducts

Heinz H. Schmeiser, Marie Stiborova, and Volker M. Arlt

Part IV: Assays in Plants and Aleternate Animal Models

22. Micronucleus Assay with Tetrad Cells of Tradescantia

Miroslav Misík, Clemens Pichler, Bernhard Rainer, Armen Nersesyan, and Siegfried Knasmueller

23. The Wing--Spot and the Comet Tests as useful Assays Detecting Genotoxicity in Drosophila

Ricard Marcos and Erico R. Carmona

Part V: Guidelines

24. Genotoxicity Guidelines Recommended by International Conference of Harmonization (ICH)

Gireesh Kamath H. and K.S. Rao