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This volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations. The chapters in this book are divided into five parts and cover subjects such as: immature oocyte cryopreservation, human sperm vitrification and slow-freezing, directional freezing of ovarian tissue, automated vitrification systems, and detailed protocols on popular and commercially available cryopreservation/vitrification systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Cryopreservation of Mammalian Gametes and Embryos: Methods and Protocols includes chapters written by leading experts in the field and is a valuable resource for anyone interested in the field of cryopreservation. 

Cryopreservation of Mammalian Gametes and Embryos: Methods and Protocols

Preface
Table of Contents
Contributing Authors

Part I. Introduction

1. Historical Background on Gamete and Embryo Cryopreservation
Jaffar Ali, Naif H AlHarbi, and Nafisa Ali

Part II. Basics and Advanced Biology
2. Utility of Animal Models for Human Ovarian Tissue CryopreservationSeul Ki Kim, Jung Ryeol Lee, and S.Samuel Kim
3. Current Challenges in Immature Oocyte CryopreservationRi-Cheng Chian, Yixin Xu, and Dana Keilty
4. Role of Antioxidants and Antifreeze Proteins in Cryopreservation/VitrificationSeul Ki Kim, Hye Won Youm, Jung Ryeol Lee, and Chang Suk Suh
Part III. Methodology
5. Slow Freezing of Human SpermAshok Agarwal and Eva Tvrda
6. Technology of Aseptic Cryoprotectant-free Vitrification of Human ICSI SpermatozoaVladimir Isachenko, Raul Sanchez, Peter Mallmann, Gohar Rahimi, and Evgenia Isachenko
7. Human Epididymal and Testicular Sperm CryopreservationPankaj Talwar and Sarabpreet Singh
8. Human Oocytes Slow-rate Freezing: MethodologyC. Zaca and A. Borini
9. Slow Freezing and Thawing of Human Cleavage Stage EmbryosDavid H. Edgar, Janell Archer, and Debra A. Gook
10. Human Oocyte VitrificationLaura Rienzi, Ana Cobo, and Filippo Maria Ubaldi
11. Human Embryo VitrificationJuergen Liebermann
12. Human Ovarian Tissue Slow FreezingDebra A. Gook
13. Human Ovarian Tissue VitrificationSherman Silber
14. Establishing an Oocyte Cryo Bank NetworkJames Graham, Josh Lim, and Michael Tucker
15. Development of a Nationwide Network for Ovarian Tissue CrypreservationJana Liebenthron and Markus Montag
Part IV. Advances in Cryotechnology, Research, and Animal Models
16. Directional Freezing of Ovarian Tissue and Freeze-drying of Stem Cells for Fertility PreservationAmir Arav and Natan Yehudit
17. Sterile Plate-Based Vitrification of Adherent Human Pluripotent Stem Cells and Their Derivatives Using the TWIST MethodJulia C. Neubauer, Frank Stracke, and Heiko Zimmermann
18. Vitrification: A Reliable Method for Cryopreservation of Animal EmbryosB. Singh, G. Mal, and S. K. Singla
19. Cryopreservation Effect on Genetic Function: Neonatal OutcomesVanesa Robles, Marta F. Riesco, and David G. Valcarce
20. Gavi-Automated Vitrification InstrumentTammie K. Roy, Susanna Brandi, and Teija Peura
Part V. Appendices
A. Cryotec® Vitrification ThawingGoral Gandhi, Masashige Kuwayama, Sakina Kagalwala, and Priyanka Pangerkar
B. Solid Surface VitrificationMohan S. Kamath and Muthukumar K.
C. Automated Vitrification of Mammalian Embryos on a Digital Microfluidic DeviceJun Liu, Derek G. Pyne, Mohamed Abdelgawad, and Yu Sun
D. Irvine Scientific®  Vitrification SystemMatthew VerMilyea and Amber Brewer
E. Rapid-i: Closed Vitrification Device by VitrolifeMark Larman
F. Quinn's Advantage Embryo Freeze KitKiri Beilby and Patrick Quinn
G. Vitrification of Blastocysts Using VitriBlast(TM) and ThermoBlast(TM) - NidaconAnna Niläng Laessker, Thorir Hardarsson, Ann-Sofie Forsberg, Tetsunori Mukaida, and Paul V. Holmes