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This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurological disorders.

Neural Stem Cells

1. Generation of Neural Stem Cells from Induced Pluripotent Stem Cells







Marcel M. Daadi















2. Making NSC and Neurons from Patient Derived Tissue Samples







Odity Mukherjee, Shubhra Acharya, and Mahendra Rao















3. Generation of definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury







Mohamad Khazaei , Christopher S. Ahuja, Christopher E. Rodgers, Priscilla Chan, and Michael G. Fehlings















4. Derivation of Neural Stem Cells from Human Parthenogenetic Stem Cells







Rodolfo Gonzalez, Ibon Garitaonandia, and Russell Kern















5. Chemically Defined Neural Conversion of Human Pluripotent Stem Cells







Yu Chen, Carlos A. Tristan, Sunil K. Mallanna, Pinar Ormanoglu, Steven Titus,







Anton Simeonov, and Ilyas Singec















6. In vitro Functional Characterization of Human Neurons and Astrocytes Using Calcium Imaging and Electrophysiology







Marita Gronning Hansen, Daniel Tornero, Isaac Canals, Henrik Ahlenius, and Zaal Kokaia















7. Differentiation of Neural Stem Cells Derived from Induced Pluripotent Stem Cells into Dopaminergic Neurons







Marcel M. Daadi















8. Reference Transcriptome for Deriving Nonhuman Primate Induced Pluripotent Stem Cells







Fabiano Tofoli de Araujo, Ana Teresa Silva Semeano, Agatha Oliveira-Giacomelli, Maria Carolina Bittencourt Goncalves, Merari de Fatima Ramires Ferrari, Lygia da Veiga Pereira,and Henning Ulrich















9. Generating Neural Stem Cells from iPSCs with Dopaminergic Neurons Reporter Gene







Hyenjong Hong and Marcel M. Daadi















10. Single-Cell Library Preparation of iPSC-derived Neural Stem Cells







Jeffrey Kim and Marcel M. Daadi















11. Bioinformatic Analysis of Single-Cell RNA-Seq Raw Data From iPSC-Derived Neural Stem Cells







Jeffrey Kim and Marcel M. Daadi















12. Assay for Assessing Mitochondrial Function In iPSC-Derived Neural Stem Cells and Dopaminergic Neurons.







Gourav R. Choudhury and Marcel M. Daadi















13. Standards for Deriving Nonhuman Primate Induced Pluripotent Stem Cells







Guang Yang, Hyenjong Hong, April Torres, Kristen E. Malloy, Gourav R. Choudhury, Jeffrey Kim, and Marcel M. Daadi















14. Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach







Steven L. Farnsworth, Zhifang Qiu, Anuja Mishra, and Peter J. Hornsby















15. Isolation and Differentiation of Self-Renewable Neural Stem Cells from Marmoset Induced Pluripotent Stem Cells







Hyenjong Hong, Gourav R. Choudhury, Jeffrey Kim, and Marcel M. Daadi















16. Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation







Cang Chen, Michael Guderyon, Guo Ge, Robert A Clark, and Senlin Li















17. Central and Peripheral Secondary Cell Death Processes After Transient Global Ischemia in Non-human Primate Cerebellum and Heart















Jea-Young Lee, Roger Lin, Hung Nguyen, Eleonora Russo, M. Grant Liska, Trenton Lippert, Yuji Kaneko and Cesar V. Borlongan















18. Method for Stimulation of Hippocampal Neurogenesis by Transient Micro-needle Insertion







Shijie Song, Xiaoyung Kong, and Juan Sanchez-Ramos