Publisher | Springer, Berlin |
Year | |
Pages | 239 |
Version | hardback |
Language | English |
ISBN | 9781493990054 |
Categories | Neurosciences |
This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurological disorders. Chapters guide the reader through methods and protocols on definition and isolation of neural stem cells, characterization and differentiation, standards for defining cell populations, and In vivo approaches in animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Neural Stem Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Neural Stem Cells: Methods and Protocols
1. Generation of Neural Stem Cells from Induced Pluripotent Stem Cells
Marcel M. Daadi
2. Making NSC and Neurons from Patient Derived Tissue Samples
Odity Mukherjee, Shubhra Acharya, and Mahendra Rao
3. Generation of definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury
Mohamad Khazaei , Christopher S. Ahuja, Christopher E. Rodgers, Priscilla Chan, and Michael G. Fehlings
4. Derivation of Neural Stem Cells from Human Parthenogenetic Stem Cells
Rodolfo Gonzalez, Ibon Garitaonandia, and Russell Kern
5. Chemically Defined Neural Conversion of Human Pluripotent Stem Cells
Yu Chen, Carlos A. Tristan, Sunil K. Mallanna, Pinar Ormanoglu, Steven Titus,
Anton Simeonov, and Ilyas Singeç
6. In vitro Functional Characterization of Human Neurons and Astrocytes Using Calcium Imaging and Electrophysiology
Marita Gronning Hansen, Daniel Tornero, Isaac Canals, Henrik Ahlenius, and Zaal Kokaia
7. Differentiation of Neural Stem Cells Derived from Induced Pluripotent Stem Cells into Dopaminergic Neurons
Marcel M. Daadi
8. Reference Transcriptome for Deriving Nonhuman Primate Induced Pluripotent Stem Cells
Fabiano Tofoli de Araújo, Ana Teresa Silva Semeano, Ágatha Oliveira-Giacomelli, Maria Carolina Bittencourt Gonçalves, Merari de Fatima Ramires Ferrari, Lygia da Veiga Pereira,and Henning Ulrich
9. Generating Neural Stem Cells from iPSCs with Dopaminergic Neurons Reporter Gene
Hyenjong Hong and Marcel M. Daadi
10. Single-Cell Library Preparation of iPSC-derived Neural Stem Cells
Jeffrey Kim and Marcel M. Daadi
11. Bioinformatic Analysis of Single-Cell RNA-Seq Raw Data From iPSC-Derived Neural Stem Cells
Jeffrey Kim and Marcel M. Daadi
12. Assay for Assessing Mitochondrial Function In iPSC-Derived Neural Stem Cells and Dopaminergic Neurons.
Gourav R. Choudhury and Marcel M. Daadi
13. Standards for Deriving Nonhuman Primate Induced Pluripotent Stem Cells
Guang Yang, Hyenjong Hong, April Torres, Kristen E. Malloy, Gourav R. Choudhury, Jeffrey Kim, and Marcel M. Daadi
14. Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach
Steven L. Farnsworth, Zhifang Qiu, Anuja Mishra, and Peter J. Hornsby
15. Isolation and Differentiation of Self-Renewable Neural Stem Cells from Marmoset Induced Pluripotent Stem Cells
Hyenjong Hong, Gourav R. Choudhury, Jeffrey Kim, and Marcel M. Daadi
16. Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation
Cang Chen, Michael Guderyon, Guo Ge, Robert A Clark, and Senlin Li
17. Central and Peripheral Secondary Cell Death Processes After Transient Global Ischemia in Non-human Primate Cerebellum and Heart
Jea-Young Lee, Roger Lin, Hung Nguyen, Eleonora Russo, M. Grant Liska, Trenton Lippert, Yuji Kaneko and Cesar V. Borlongan
18. Method for Stimulation of Hippocampal Neurogenesis by Transient Micro-needle Insertion
Shijie Song, Xiaoyung Kong, and Juan Sanchez-Ramos