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Plant Long Non-Coding RNAs

Plant Long Non-Coding RNAs

Publisher Springer Nature Customer Service Center GmbH
Year 01/01/2019
Edition First
Pages 480
Version hardback
Readership level Professional and scholarly
Language English
ISBN 9781493990443
Categories Genetics (non-medical), Molecular biology, Plant physiology
$196.73 (with VAT)
762.00 PLN / €166.10 / £150.10
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Book description

This volume focuses on various approaches to studying long non-coding RNAs (lncRNAs), including techniques for finding lncRNAs, localization, and observing their functions. The chapters in this book cover how to catalog lncRNAs in various plant species; determining subcellular localization; protein interactions; structures; and RNA modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and innovative, Plant Long Non-Coding RNAs: Methods and Protocols is a valuable resource that aids researchers in understanding the functions of lncRNAs in different plant species, and helps them explore currently uncharted facets of plant biology.

Plant Long Non-Coding RNAs

Table of contents

Table of Contents...
Contributing Authors...

1An Overview of Methodologies in Studying lncRNAs in the High-Throughput Era: When Acronyms ATTACK!
Hsiao-Lin V. Wang and Julia A. Chekanova

Part I Role of ncRNAs in Regulation of Gene Expression and Development in Plants

2Analysis of siRNA Precursors Generated by RNA Polymerase IV and RNA-Dependent RNA Polymerase 2 in Arabidopsis
Todd Blevins, Ram Podicheti, and Craig S. Pikaard

3Identification of Long Non-Coding RNAs in the Developing Endosperm of Maize
Eundeok Kim, Yuqing Xiong, Byung-Ho Kang, and Sibum Sung 

4RNA Isolation and Analysis of lncRNAs from Gametophytes of Maize
Linqian Han, Lin Li, Gary J. Muehlbauer, John E. Fowler, and Matthew M. S. Evans

Part II Studying the Tissue and Cell-Type Specific lncRNAs

5Improved Method of RNA Isolation from Laser Capture Microdissection (LCM) Derived-Plant Tissues
Vibhav Gautam, Archita Singh, Sharmila Singh, Swati Verma, and Ananda K. Sarkar

6Medium Throughput RNA In Situ Hybridization of Serial Sections from Paraffin-Embedded Tissue Microarrays
Edith Francoz, Philippe Ranocha, Christophe Dun, and Vincent Burlat

7Purification and Functional Analysis of Plant Long Non-Coding RNAs (lncRNA)
Trung Do, Zhipeng Qu, and Iain Searle

Part III Studying lncRNAs Associated with Response to Abiotic and Biotic Stresses in Plants 

8The Involvement of Long Non-Coding RNAs in Response to Plant Stress
Akihiro Matsui and Motoaki Seki

9Subcellular Localization and Functions of Plant lncRNAs in Drought and Salt Stress Tolerance
Tao Qin and Liming Xiong

10Discovery, Identification, and Functional Characterization of Plant Long Intergenic Non-Coding RNAs after Virus Infection
Ruimin Gao, Peng Liu, Nadia Irwanto, De Rong Loh, and Sek-Man Wong

Part IV Identification and Functional Analysis of lncRNAs

11Bioinformatics Approaches to Studying Plant Long Non-Coding RNAs (lncRNAs): Identification and Functional Interpretation of lncRNAs from RNA-seq Data Sets
Hai-Xi Sun and Nam-Hai Chua

12Identification of Novel lincRNA and Co-Expression Network Analysis using RNA Sequencing Data in Plants
Song Qi, Shamima Akter, and Song Li

13An Easy-to-Follow Pipeline for Long Non-Coding RNA Identification: A Case Study in Diploid Strawberry Fragaria vesca
Chunying Kang and Zhongchi Liu

14Reference-Based Identification of Long Non-Coding RNAs in Plants with Strand-Specific RNA-Sequencin

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