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Unique in this field, Protocols for Semen Analysis in Clinical Diagnosis defines basic semen analysis concepts, provides clinically essential reference values, and elucidates the often subtle relationships between many sperm characteristics and their relevance for clinical diagnosis. Dr. Jeyendran takes readers logically and systematically through each step of the diagnostic process. He discusses the underlying rationale for implementing different kinds of semen analysis, the intricacies of routine versus specialized testing, and additional diagnostic testing. The book provides readers with easy to use procedural protocols and a comprehensive reference.

Protocols for Semen Analysis in Clinical Diagnosis

Preface Introduction 1. Sperm Procurement Techniques 2. Semen Collection 3. Factors Affecting Ejaculate Counts 4. Biological Foundation for Specific Semen Assays 5. Questions and Answers 6. Semen Variable Designation 7. Semen Analysis and Sperm Assays 8. Can Sperm Get to the Fertilization Site? 9. Macroscopic Analysis 10. Microscopic Analysis 11. Sperm Mucus Penetration Test 12. Specialized Semen Analysis 13. Specialized Tests: When Routine Results are 'Normal' 14. Specialized Tests: When Routine Results are 'Abnormal' 15. Optional Clinical Semen Analysis 16. Sperm Nucleus Integrity Assay 17. Reactive Oxygen Species 18. Sperm Acrosin Assay 19. Creatine Phosphokinase Activity 20. Quality Control and Quality Assurance 21. The Need for Quality Control 22. External and Internal Quality Control 23. Reliability and Repeatability 24. Quality Control and Quality Assurance 25. Quality Assurance: The Bottom Line 26. Diagnostic Semen Analysis 27. Optimizing Fertility Treatment 28. Reporting Results 29. End Goal: Restoration of Natural Fertilization Potential 30. Post script 31. Suggested Additional Reading Appendices i. Sample Collection Instructions and Patient Questionnaire ii. Media and Protein Supplementation iii. Leukocyte Detection Methods iv. Diff-Quik Sperm Morphology Staining Method v. In vitro Sperm Capacitation Induction vi. In vitro Sperm Capacitation Enhancement vii. Quality Control for Chemical Analysis