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Meeting the need for an up-to-date and detailed primer on all aspects of the topic, this ready reference reflects the incredible expansion in the application of FRET and its derivative techniques over the past decade, especially in the biological sciences. This wide diversity is equally mirrored in the range of expert contributors.The book itself is clearly subdivided into four major sections. The first provides some background, theory, and key concepts, while the second section focuses on some common FRET techniques and applications, such as in vitro sensing and diagnostics, the determination of protein, peptide and other biological structures, as well as cellular biosensing with genetically encoded fluorescent indicators. The third section looks at recent developments, beginning with the use of fluorescent proteins, followed by a review of FRET usage with semiconductor quantum dots, along with an overview of multistep FRET. The text concludes with a detailed and greatly updated series of supporting tables on FRET pairs and Förster distances, together with some outlook and perspectives on FRET. Written for both the FRET novice and for the seasoned user, this is a must-have resourcefor office and laboratory shelves.

FRET - Förster Resonance Energy Transfer: From Theory to Applications

PrefacePART ONE: BACKGROUND, THEORY, AND CONCEPTSHow I Remember Theodor FörsterRemembering Robert Clegg and Elizabeth Jares-Erijman and Their Contributions to FRETFörster TheoryOptimizing the Orientation Factor Kappa-Squared for More Accurate FRET MeasurementsHow to Apply FRET: From Experimental Design to Data AnalysisMaterials for FRET Analysis: Beyond Traditional Dye-Dye CombinationsPART TWO: COMMON FRET TECHNIQUES/APPLICATIONSIn Vitro FRET Sensing, Diagnostics, and Personalized MedicineSingle-Molecule ApplicationsImplementation of FRET Technologies for Studying the Folding and Conformational Changes in Biological StructuresFRET-Based Cellular Sensing with Genetically Encoded Fluorescent IndicatorsPART THREE: FRET WITH RECENTLY DEVELOPED MATERIALSFRET with Fluorescent ProteinsSemiconductor Quantum Dots and FRETMultistep FRET and NanotechnologyPART FOUR: SUPPORTING INFORMATION AND CONCLUSIONSDataOutlook on FRET: The Future of Resonance Energy TransferIndex