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How can I use my HPLC/UHPLC equipment in an optimal way, where are the limitations of the technique? These questions are discussed in detail in the sequel of the successful "HPLC Expert" in twelve chapters written by experts in the respective fields. The topics encompass - complementary to the first volume - typical HPLC users' problems and questions such as gradient optimization and hyphenated techniques (LC-MS). An important key aspect of the book is UHPLC: For which analytical problem is it essential, what should be considered? Besides presentation of latest developments directly from the main manufacturers, also UHPLC users and independent service engineers impart their knowledge. Consistent with the target groups, the level is advanced, but the emphasis is on practical applications.
The HPLC Expert II: Find and Optimize the Benefits of your HPLC/UHPLC
Introduction: When should I use my UHPLC as a UHPLC? PART 1: HARDWARE AND SOFTWARE, SEPARATION MODES, MATERIALSModern HPLC/UHPLC equipmentRequirements for the different parts of HPLC/UHPLC equipmentThe column oven - a simple matter?The problem of the extra column dispersion in an HPLC/UHPLC equipmentThe gradient: Requirements, optimal operation, tricks and pitfallsHardware requirements for LC coupling with different mass spectrometers2D-Chromatography - potentials and limitations Selecting the right column materials in HPLC and UHPLCQality by Design in HPLC Method DevelopmentPART 2: USE CASES AND TRENDSWhat should the software be able to do, so that the hardware can be used to its full potential?Aspects of modern HPLC - a user´s experience reportHints and recommendations for optimal use of HPLC equipmentUsing UHPLC to solve the compound separation problemEquipment review (Agilent, Shimadzu, ThermoScientific)