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Sample Preparation in LC-MS Bioanalysis

Sample Preparation in LC-MS Bioanalysis

Autorzy
Wydawnictwo Blackwell Science
Data wydania 01/02/2019
Wydanie Pierwsze
Liczba stron 384
Forma publikacji książka w twardej oprawie
Poziom zaawansowania Dla profesjonalistów, specjalistów i badaczy naukowych
Język angielski
ISBN 9781119274292
Kategorie Biologia
716.00 PLN (z VAT)
$189.16 / €157.22 / £135.82 /
Produkt dostępny
Dostawa 2 dni
Ilość
Do schowka

Opis książki

Revised and Expanded Handbook Provides Comprehensive Introduction and Complete Instruction for Sample Preparation in Vital Category of Bioanalysis Following in the footsteps of the previously published Handbook of LC-MS Bioanalysis, this book is a thorough and timely guide to all important sample preparation techniques used for quantitative Liquid Chromatography-Mass Spectrometry (LC-MS) bioanalysis of small and large molecules. LC-MS bioanalysis is a key element of pharmaceutical research and development, post-approval therapeutic drug monitoring, and many other studies used in human healthcare. While advances are continually being made in key aspects of LC-MS bioanalysis such as sensitivity and throughput, the value of research/study mentioned above is still heavily dependent on the availability of high-quality data, for which sample preparation plays the critical role. Thus, this text provides researchers in industry, academia, and regulatory agencies with detailed sample preparation techniques and step-by-step protocols on proper extraction of various analyte(s) of interest from biological samples for LC-MS quantification, in accordance with current health authority regulations and industry best practices. The three sections of the book with a total of 26 chapters cover topics that include: Current basic sample preparation techniques (e.g., protein precipitation, liquid-liquid extraction, solid-phase extraction, salting-out assisted liquid-liquid extraction, ultracentrifugation and ultrafiltration, microsampling, sample extraction via electromembranes) Sample preparation techniques for uncommon biological matrices (e.g., tissues, hair, skin, nails, bones, mononuclear cells, cerebrospinal fluid, aqueous humor) Crucial aspects of LC-MS bioanalytical method development (e.g., pre-analytical considerations, derivation strategies, stability, non-specific binding) in addition to sample preparation techniques for challenging molecules (e.g., lipids, peptides, proteins, oligonucleotides, antibody-drug conjugates) Sample Preparation in LC-MS Bioanalysis will prove a practical and highly valuable addition to the reference shelves of scientists and related professionals in a variety of fields, including pharmaceutical and biomedical research, mass spectrometry, and analytical chemistry, as well as practitioners in clinical pharmacology, toxicology, and therapeutic drug monitoring. "A welcome reference which provides a complete overview of current and new approaches in sample preparation for LC-MS...To have an

Sample Preparation in LC-MS Bioanalysis

Spis treści

List of Contributors xvi


Preface xx


List of Abbreviations xxii


Part I Current Sample Preparation Techniques in LC?MS Bioanalysis 1


1 Basic Sample Preparation Techniques in LC?MS Bioanalysis: Protein Precipitation, Liquid-Liquid Extraction, and Solid?Phase Extraction 3
Wenkui Li, Wenying Jian, and Yunlin Fu


1.1 Introduction 3


1.2 Physicochemical Properties of Drugs and Their Metabolites 4


1.3 Pre?analytical Variables of Analyte(s) of Interest in Biological Matrix 5


1.4 Most Commonly Used Sample Preparation Methods in LC?MS Bioanalysis 7


References 27


2 Online Extraction and Column Switching Techniques in LC?MS Bioanalysis 31
Yan Mao and Mike (Qingtao) Huang


2.1 Introduction 31


2.2 System Configuration 32


2.3 Commonly Used Online Extraction Techniques 33


2.4 Considerations for Utilizing Online Extraction Techniques 40


2.5 Summary 41


References 42


3 Equilibrium Dialysis, Ultracentrifugation, and Ultrafiltration in LC?MS Bioanalysis 45
Theo de Boer and Henri Meijering


3.1 Introduction 45


3.2 Challenges and Considerations 46


3.3 Experimental Procedures 46


3.4 Summary 49


References 51


4 Phospholipid Depletion Techniques in LC?MS Bioanalysis 52
Stacy Brown and Jennifer Carmical


4.1 Introduction 52


4.2 Impact of Phospholipids on Bioanalytical Methods 52


4.3 Investigating Matrix Effects Associated with Phospholipids 55


4.4 Minimizing Matrix Effects Associated with Phospholipids 56


4.5 Removing Phospholipids Prior to LC?MS Analysis 57


4.6 Example Methods that Demonstrate Successful Phospholipid Removal 60


4.7 Conclusions 63


Acknowledgement 64


References 64


5 Salting?out Assisted Liquid-Liquid Extraction (SALLE) in LC?MS Bioanalysis 68
Jun Zhang and Xin Xiong


5.1 Introduction 68


5.2 Considerations in Developing a SALLE Method 68


5.3 Combination of SALLE with Other Extraction Techniques 72


5.4 Matrix Effect in SALLE 72


5.5 Miniaturization and Automatization 73


5.6 Summary 73


References 73


6 Supported Liquid Extraction (SLE) in LC?MS Bioanalysis 76
Zhongzhe Cheng and Hongliang Jiang


6.1 Introduction 76


6.2 Principle of SLE 76


6.3 Advantages and Limitation of SLE in Quantitative LC?MS Bioanalysis 77


6.4 Key Consideration in Developing Robust SLE?LC?MS Bioanalytical Method 79


6.5 Representative Protocols 80


6.6 Summary 81


References 82


7 Immunocapture in LC?MS Bioanalysis 85
Ang Liu


7.1 Introduction 85


7.2 Experimental Workflow and Optimization 85


7.3 Considerations on the Selection of Capture Reagents and the Limitatio

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